Anxiety treatment
Does cannabigerol calm rats via serotonin receptors?
Open access · cc by · source: Europe PMC
In 176 male rats, cannabigerol weakened α2 and 5-HT1A agonist suppression of LC/DRN firing in slices and reduced anxiety-like behavior in plus-maze and novelty feeding—effects blocked by WAY100635.
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Study at a glance
- Design
- Animal / in-vitro — Male Sprague-Dawley rat LC/DRN slice electrophysiology plus elevated plus maze and novelty-suppressed feeding, with WAY100635 challenge
- N
- N=176 · 176 rats total: 118 behavioral, 58 electrophysiology
- Population
- Male Sprague-Dawley rats (200–300 g); locus coeruleus and dorsal raphe nucleus slices plus in vivo anxiety tests
- Outcome
- NA and 5-HT neuron firing modulation by α2 and 5-HT1A agonists, plus anxiolytic-like EPMT/NSFT behavior
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Does cannabigerol calm rats via serotonin receptors?
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
176 male Sprague-Dawley rats were used (118 behavioral; 58 electrophysiology).
Male Sprague-Dawley (200–300 g) rats (total, n = 176; behavioral tests, n = 118; and electrophysiological assays, n = 58)
CBG was tested at 30 μM for 10 min in slices.
CBG (30 μM, 10 min) slightly changed the firing rate of NA cells but failed to alter the inhibitory effect of NA (1–100 µM)
Key findings
CBG barely changed baseline firing and did not block NA or 5-HT itself, but reduced inhibition by UK14304 and ipsapirone. WAY100635 restored firing after ipsapirone; CBG did not. In vivo, CBG increased open-arm time and cut feeding latency, prevented by WAY100635.
Methodology
Recorded locus coeruleus NA and dorsal raphe 5-HT cell firing in slices with CBG (30 μM) versus NA, UK14304, 5-HT, ipsapirone, and WAY100635, and tested CBG 10 mg/kg i.p. in elevated plus maze and novelty-suppressed feeding with a 5-HT1A antagonist pretreatment.
Limitations
Rat slice and maze tests do not establish human anxiolysis or a direct receptor binding mechanism; authors call the slice effect an unknown indirect action.
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