CRISPR repeats conserve RNA-like structure
CRISPR repeats across microbes form clusters with conserved sequence and predicted RNA secondary structure.
Source
Evolutionary conservation of sequence and secondary structures in CRISPR repeats
Study at a glance
- Design
- Computational / modelling — Genome-wide CRISPR array detection and repeat secondary-structure scoring
- N
- N=439 · 439 bacterial/archaeal genomes; 561 arrays in 195 genomes
- Population
- Bacterial and archaeal sequenced genomes
- Outcome
- Conserved CRISPR repeat sequence/structure features across clusters
Structured fields used in claim comparison tables when every cited study has a complete layer.
What they did
Scanned 439 genomes for CRISPR arrays and folded repeats to compare structure scores across clusters.
What they found
561 arrays in 195 genomes; some repeat clusters show consistently high folding potential supporting RNA intermediates.
The limits
What it doesn't show
Structure is computational prediction, not direct RNA structure proof for every array.
Key terms
- CRISPR repeat
- Short repeated sequence flanking spacers.
- Spacer
- Unique insert often from invaders.
- CAS genes
- CRISPR-associated protein families.
- RNA fold
- Predicted secondary structure.
- PILER-CR
- CRISPR array finder.
- Horizontal transfer
- Movement of CRISPR systems between lineages.
Flashcards
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Common questions
Genomes scanned?
439.
Arrays?
561.
Genomes with CRISPR?
44%.
Focus?
Repeat structure.
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