Gene expression
Which follicle genes differ in high- vs low-laying hens?
Open access · cc by · source: Europe PMC
RNA-seq of 18 follicle libraries from 10 high-laying Lohmann Brown and 10 low-laying Jilin Black hens found 236/544/386 DEGs at LWF/SYF/LYF stages, 13 genes shared across stages, and KEGG hits including neuroactive ligand–receptor and cAMP signaling.
How this study connects
Role on claims
Each row is a claim on a concept or method page where this paper supports, challenges, or qualifies the statement. Roles are hand-checked — not a model guess.
Not yet placed on a claim. This paper has study layers, but no concept page yet cites it as support, challenge, or qualifier.
Study at a glance
- Design
- Animal / in-vitro — RNA-seq of LWF/SYF/LYF ovarian follicles from high- vs low-laying hens (Lohmann Brown vs Jilin Black); 18 libraries, DEG and KEGG analysis
- N
- N=20 · 10 hens per breed (LB high-laying vs JB low-laying) at 21 weeks; 18 cDNA libraries across three follicle stages
- Population
- Lohmann Brown (HR) and Jilin Black (LR) laying hens, ovarian follicles at 21 weeks
- Outcome
- Differentially expressed unigenes and pathways linked to egg-production rate
Structured fields used in claim comparison tables when every cited study has a complete layer.
Inspect source passages
Which follicle genes differ in high- vs low-laying hens?
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
DEG counts were 236, 544, and 386 in LWF, SYF, and LYF.
Eighteen cDNA libraries were constructed and a total of 236, 544, and 386 unigenes were significantly differentially expressed in the LWF, SYF, and LYF follicles of HR and LR hens, respectively.
21-week laying rates were 53% (LB) vs 11% (JB).
average egg production rate at the age were 53 and 11%, respectively
Key findings
236, 544, and 386 unigenes differed in LWF, SYF, and LYF. Thirteen DEGs were co-expressed in all three stages; 18 candidates (P2RX1, CAB39L, BLK, and others) were tied to laying. Pathways included neuroactive ligand–receptor interaction, cell adhesion molecules, PPAR, and cAMP signaling.
Methodology
Harvested LWF, SYF, and LYF follicles at 21 weeks from 10 birds per breed (egg production ~53% vs 11%), built 18 Illumina libraries, called DEGs (padj < 0.05, |log2FC| > 1), and annotated with GO/KEGG plus RT-qPCR of candidates.
Limitations
Bulk follicle RNA-seq in two breeds cannot prove any single DEG causes laying-rate differences, and 10 hens/breed is a small genetic sample.
Related papers in this topic
Same topic cluster — not a recommendation engine.