Gene expression
Vascularized brain organoids tracked with scRNA-seq
Open access · cc by · source: Europe PMC
Human cortical vOrganoids kept for >200 days show cortical cell types, vessel-like structure, synapses, and scRNA-seq neurogenesis/vessel programs; grafts form functional human–mouse vessels in mouse S1 cortex.
Study at a glance
- Design
- Animal / in-vitro — Vascularized human cortical organoids with electrophysiology, scRNA-seq, and mouse S1 transplant
- N
- Organoid cultures maintained >200 days — no single primary analytic N in stored text
- Population
- Human cortical organoids (vOrganoids) and mouse graft hosts
- Outcome
- Vascularization, synapses, and graft–host vessel formation in cortical organoids
Structured fields used in claim comparison tables when every cited study has a complete layer.
Key findings
vOrganoids contained typical human cortical cell types and a vascular structure for over 200 days. Synaptic assays indicated chemical and electrical synapses. scRNA-seq showed robust neurogenesis and differentially expressed genes related to blood-vessel morphogenesis. After transplant into mouse S1 cortex, functional human–mouse blood vessels formed in grafts and promoted cell survival. Authors argue vasculature reduces hypoxia/cell death and promotes neural development in organoids.
Methodology
Authors generated vascularized human cortical organoids (vOrganoids), recorded spontaneous excitatory and inhibitory synaptic currents plus bidirectional electrical transmission, profiled cells with single-cell RNA-seq, and transplanted organoids into mouse primary somatosensory (S1) cortex to test graft–host vessels and cell survival.
Limitations
This is a developmental and transplant model, not a finished clinical cell therapy. Graft vessels form in a mouse host cortex, so host biology shapes the reconstructed vasculature. Disease-modeling claims are prospective; the paper does not prove treatment of human nervous-system injury.
How this study connects
Role on claims
Each row is a claim on a concept or method page where this paper supports, challenges, or qualifies the statement. Roles are hand-checked — not a model guess.
scRNA-seq can separate definitive endoderm progenitors and point to hypoxia effects in hESC cultures.
In human ES differentiation, scRNA-seq separated definitive endoderm progenitors (PC5 signature) and motivated severe hypoxia experiments that increased CXCR4+ DE-like cells.
Scope note — different system — cortical organoids, not hESC endoderm
Limits the claim's scope: a different population, assay, or outcome.
In vascularized cortical organoids, scRNA-seq tracks neurogenesis and vessel-related programs.
Single-cell RNA-seq of vOrganoids illustrated robust neurogenesis and differentially expressed genes related to blood-vessel morphogenesis, alongside synaptic physiology and transplant vessel formation.
Evidence for the claim as stated.
A CRC single-cell atlas links CMS labels to immune/stromal states and outcomes.
Across ~487,829 cells, tumor cells recapitulated CMS subgroups with intratumoral heterogeneity; MSI-H tumors showed CD8 cytotoxic infiltration patterns; high CAF and C1Q+ TAM content associated with poorer outcomes.
Scope note — different tissue — brain organoid model, not CRC
Limits the claim's scope: a different population, assay, or outcome.
Method papers and toolboxes in the wider queue are not interchangeable with these empirical atlases; this page only cites studies that make a biological claim with single-cell profiles.
Evidence for the claim as stated.
Open questions
Tensions this paper is part of
From concept pages' “where studies disagree.” Disagreement means the same question; scope means different assays, populations, or outcomes.
Method papers and toolboxes in the wider queue are not interchangeable with these empirical atlases; this page only cites studies that make a biological claim with single-cell profiles.
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