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Do pericytes help the brain recover after a stroke?

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In PDGFRβ-deficient mice after permanent MCAO, weaker pericyte-driven infarct repair tracked with less peri-infarct astrogliosis, poorer oligodendrogenesis/remyelination, and worse functional recovery.

Source

Pericyte-Mediated Tissue Repair through PDGFRβ Promotes Peri-Infarct Astrogliosis, Oligodendrogenesis, and Functional Recovery after Acute Ischemic Stroke

Shibahara T, Ago T, Nakamura K, et al. · eNeuro · 2020

doi.org/10.1523/eneuro.0474-19.2020Read the full paper ↗70 citationscc by

What they did

They compared repair and peri-infarct remodeling after pMCAO in Pdgfrb+/– mice and tested how PDGF-BB–treated pericyte medium affects astrocytes and OPC differentiation in culture.

What they found

Repair, CBF recovery, STAT3-linked astrogliosis, OPC differentiation/remyelination, and functional recovery were impaired when pericyte PDGFRβ signaling was reduced; PCM/PDGF-BB boosted astrocyte activity and ACM+PCM/PDGF-BB enhanced myelination.

The limits

What it doesn't show

Mouse permanent-occlusion findings do not prove that boosting PDGFRβ is a safe human stroke therapy.

Key terms

PDGFRβ
Receptor on pericytes central to post-stroke fibrotic repair.
Pericyte
Vessel-associated cell that helps stabilize microvasculature and scar formation.
pMCAO
Permanent middle cerebral artery occlusion stroke model.
Astrogliosis
Reactive astrocyte response around injured brain tissue.
OPC
Oligodendrocyte precursor cell that can remyelinate axons.
Oligodendrogenesis
Generation/differentiation of oligodendrocytes after injury.

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Stroke model:

Common questions

Model?

Permanent MCAO in Pdgfrb+/– mice.

What failed in mutants?

Infarct size reduction, CBF recovery, astrogliosis, OPC differentiation/remyelination.

In vitro clue?

PCM/PDGF-BB activated STAT3 in astrocytes; ACM+PCM/PDGF-BB boosted OPC myelination.

Functional link?

Recovery correlated with repair and peri-infarct oligodendrogenesis.

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