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Can docking find new NMDA blockers for Alzheimer’s?

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A pharmacophore-docking-MD pipeline screened ZINC against GluN2B NMDA receptors and nominated six compounds with better predicted solvation binding than ifenprodil—still untested in cells or people.

Source

Targeting NMDA receptor in Alzheimer's disease: identifying novel inhibitors using computational approaches

Siddiqui AJ, Badraoui R, Jahan S, et al. · Frontiers in pharmacology · 2023

doi.org/10.3389/fphar.2023.1208968Read the full paper ↗21 citationscc by

Study at a glance

Design
Computational / modelling — Pharmacophore validation (20 known NMDA inhibitors + 380 DUDE decoys), ZINC virtual screen, Glide docking, MD/solvation free energy vs ifenprodil
N
N=6 · Six ZINC leads after 38,105 pharmacophore hits; pharmacophore test set 20 actives + 380 inactives
Population
In silico GluN2B/NMDAR ligands from ZINC and PDB ifenprodil-bound complexes; no new wet-lab or patient dosing
Outcome
Predicted binding (GlideScore, solvation free energy, ADMET) of six candidate Gly/NMDA antagonists versus ifenprodil

Structured fields used in claim comparison tables when every cited study has a complete layer.

What they did

Built a pharmacophore from known NMDAR inhibitors, validated it on 20 actives plus 380 DUDE decoys, filtered ZINC (38,105 hits), docked to ifenprodil-bound NMDAR with Glide, then ranked ADMET and solvation free energies.

What they found

Six ZINC molecules (including ZINC13729211 and ZINC07430424) showed high predicted affinity, hydrogen bonding, stability, and better solvation-based affinity than ifenprodil with acceptable ADMET; authors propose them as Gly/NMDA antagonist starting points.

The limits

What it doesn't show

No enzyme, cell, animal, or Alzheimer-patient data; docking and MM-PBSA cannot prove clinical NMDA blockade or safety.

Key terms

NMDAR
Ionotropic glutamate receptor; extrasynaptic overactivation is linked to Alzheimer excitotoxicity.
GluN2B
NMDAR subunit; allosteric GluN2B inhibitors are a major targeting strategy.
Ifenprodil
Prototype GluN2B-selective allosteric inhibitor used as docking/reference ligand.
Pharmacophore
3D feature model used to filter ZINC; validated with 20 known inhibitors + 380 decoys.
GlideScore
Docking score plus key residue contacts used to pick hits.
ADMET
Predicted absorption, distribution, metabolism, excretion, and toxicity filters.

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Common questions

How many leads were nominated?

Six ZINC compounds with high predicted affinity.

How large was the pharmacophore hit list?

38,105 ZINC molecules after pharmacophore filtering.

What was the reference drug?

Ifenprodil, a GluN2B-selective allosteric inhibitor.

Were the compounds tested in animals?

No—the authors say they still need in vitro/in vivo tests.

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