Polymers
pH-switchable polymer that pulls down N-glycopeptides
Open access · cc by · source: Europe PMC
A soluble poly(acrylic acid-co-hydrazide) captures glycopeptides in homogeneous solution and precipitates them by dropping pH for MS.
How this study connects
Role on claims
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This library holds 5 empirical chemistry papers on polymers with isolated findings, rates or spectra rather than reviews.
Evidence for the claim as stated.
A soluble poly(acrylic acid-co-hydrazide) captures glycopeptides in homogeneous solution and precipitates them by dropping pH for MS.
Evidence for the claim as stated.
A lab-scale ATRP or a DFT interface study does not by itself prove processability or lifetime in use.
Evidence for the claim as stated.
Open questions
Tensions this paper is part of
From concept pages' “where studies disagree.” Disagreement means the same question; scope means different assays, populations, or outcomes.
A lab-scale ATRP or a DFT interface study does not by itself prove processability or lifetime in use.
Study at a glance
- Design
- Other — Homogeneous hydrazide polymer enrichment of periodate-oxidized N-glycopeptides for LC-MS
- N
- Method development; mouse-brain application identified 1,317 N-glycopeptides / 458 glycoproteins
- Population
- Model glycoproteins and mouse-brain digests
- Outcome
- Enrichment efficiency and N-glycoproteome identification performance
Structured fields used in claim comparison tables when every cited study has a complete layer.
Inspect source passages
pH-switchable polymer that pulls down N-glycopeptides
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
Fast enrichment with >95% efficiency can be achieved within 1 h.
First, fast enrichment with >95% efficiency can be achieved within 1 h due to the unrestricted mass transfer in the homogeneous enrichment system.
Signal intensity and S/N of the 1741.8 m/z glycopeptide increase 29 and 325 times after enrichment.
For example, the signal intensity and S / N of the glycopeptide at 1741.8 m / z increases 29 and 325 times after enrichment, respectively.
Key findings
Homogeneous capture exceeds 95% within 1 h. Asialofetuin recovery is ~90%; a 1741.8 m/z glycopeptide gains 29× intensity and 325× S/N. Mouse brain gave 1317 non-redundant N-glycopeptides and 458 glycoproteins (80% in ≥2 replicates) with 1.46% FDR.
Methodology
Authors copolymerized methyl acrylate and acrylic acid (1:8), converted esters to hydrazides, periodate-oxidized glycans, captured aldehydes in solution, cycled pH to wash, released peptides with PNGase F, and identified sites by LC-MS/MALDI.
Limitations
The method uses mouse brain, not a clinical plasma cohort; periodate chemistry destroys some glycan structural information, and false deamidation still occurs at 1.46%.
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