Mass spectrometry
Screening reactions in one-second DESI-MS droplets
Open access · cc by · source: Europe PMC
Desorption electrospray ionization mass spectrometry reads amine alkylation and Suzuki hits from nanoliter spots in about a second.
How this study connects
Role on claims
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Desorption electrospray ionization mass spectrometry reads amine alkylation and Suzuki hits from nanoliter spots in about a second.
Evidence for the claim as stated.
Discoveries this paper informs or conflicts with
- Six thousand reactions an hour changes which chemistry questions can be asked
This paper informs this development.
Study at a glance
- Design
- Animal / in-vitro — DESI-MS readout of nanoliter N-alkylation and Suzuki spots on PTFE plates
- N
- Up to 6,004 reactions per hour at 6,144-well density; <0.5 μg reagent per 50 nL spot
- Population
- Miniaturized organic reaction mixtures
- Outcome
- DESI-MS screens amine alkylations and Suzuki couplings in about a second per droplet
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Inspect source passages
Screening reactions in one-second DESI-MS droplets
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
DESI-MS analyses product outcomes in reaction droplets within about 1 s.
In this paper, we describe an MS method that analyses product outcomes in reaction droplets within about 1 s.
Alkylation succeeded for 7 of 8 amines with benzyl bromide and 3 of 8 with 2-(bromoethyl)benzene.
The reaction was successful for 7 out of 8 amines with benzyl bromide and 3 out of 8 amines with 2-(bromoethyl)benzene, the difference reflecting the known difference in bromoalkane reactivity.
Key findings
Product ions appear within about 1 s per droplet. Benzyl bromide alkylated 7 of 8 amines versus 3 of 8 for 2-(bromoethyl)benzene. Peak analysis reached 6004 reactions per hour at 6144-well density, using less than 0.5 μg of each reagent in 50 nL spots.
Methodology
Authors spotted nanoliter reaction mixtures on porous PTFE, analyzed them by DESI-MS/MS, and screened N-alkylations plus Pd-catalyzed Suzuki couplings across multi-thousand-spot plates.
Limitations
Qualitative reproducibility falls at the highest speeds, and translating every DESI hit to optimized continuous-flow yield is not completed in this paper.
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