Mass spectrometry
Screening reactions in one-second DESI-MS droplets
Open access · cc by · source: Europe PMC
Desorption electrospray ionization mass spectrometry reads amine alkylation and Suzuki hits from nanoliter spots in about a second.
Key findings
Product ions appear within about 1 s per droplet. Benzyl bromide alkylated 7 of 8 amines versus 3 of 8 for 2-(bromoethyl)benzene. Peak analysis reached 6004 reactions per hour at 6144-well density, using less than 0.5 μg of each reagent in 50 nL spots.
Methodology
Authors spotted nanoliter reaction mixtures on porous PTFE, analyzed them by DESI-MS/MS, and screened N-alkylations plus Pd-catalyzed Suzuki couplings across multi-thousand-spot plates.
Limitations
Qualitative reproducibility falls at the highest speeds, and translating every DESI hit to optimized continuous-flow yield is not completed in this paper.
How this study connects
Role on claims
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Desorption electrospray ionization mass spectrometry reads amine alkylation and Suzuki hits from nanoliter spots in about a second.
Evidence for the claim as stated.
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