Diagnostic accuracy
Rapid gram-positive blood culture ID
Open access · cc by · source: Europe PMC
A microarray panel identified Staphylococcus from positive blood cultures with ~99% sensitivity and specificity versus culture.
Study at a glance
- Design
- Other — Prospective diagnostic accuracy study of automated microarray panel vs reference culture
- N
- N=1157 · 1,157 prospectively collected monomicrobial positive blood cultures
- Population
- Monomicrobial positive blood cultures
- Outcome
- Sensitivity and specificity for gram-positive organisms and resistance markers
Structured fields used in claim comparison tables when every cited study has a complete layer.
Key findings
Among 1,157 monomicrobial cultures, Staphylococcus genus sensitivity was 99.4% and specificity 99.7%; staphylococci dominated the culture set.
Methodology
Prospective monomicrobial positive blood cultures were tested with an automated microarray nucleic-acid panel for gram-positive organisms and resistance markers against reference culture.
Limitations
Performance varies by organism/target; this evaluates assay accuracy, not downstream mortality benefit of faster reporting.
How this study connects
Role on claims
Each row is a claim on a concept or method page where this paper supports, challenges, or qualifies the statement. Roles are hand-checked — not a model guess.
A multiplex assay can identify Staphylococcus from blood cultures with very high accuracy.
A multiplex molecular assay on monomicrobial blood cultures identified Staphylococcus at genus level with sensitivity about 99.4% and specificity about 99.7% in a large evaluated set.
Evidence for the claim as stated.
An automated microarray panel for gram-positive organisms and resistance markers on 1,157 monomicrobial positive blood cultures reached Staphylococcus genus sensitivity 99.4% and specificity 99.7% versus reference culture. Assay accuracy is not a mortality benefit of faster reporting, and performance varies by organism and target.
Evidence for the claim as stated.
The five papers are tuned to different prevalences and harms of error, so a 'good' sensitivity/specificity pair is not portable. CA125's 77%/93.8% at 0.9% incidence yields PPV 10.1% — a primary-care rare-disease problem. StEP's 95%/93% pinprick figures come from specialist clinics where neuropathic pain is common. FibriCheck's 95.6%/96.6% is versus ECG after dropping poor signals. Blood-culture 99.4%/99.7% is laboratory identification, not community screening. Frailty's 99.1% specificity describes a ~1.1% trajectory class, not a test you run on everyone.
Evidence for the claim as stated.
Open questions
Tensions this paper is part of
From concept pages' “where studies disagree.” Disagreement means the same question; scope means different assays, populations, or outcomes.
The five papers are tuned to different prevalences and harms of error, so a 'good' sensitivity/specificity pair is not portable. CA125's 77%/93.8% at 0.9% incidence yields PPV 10.1% — a primary-care rare-disease problem. StEP's 95%/93% pinprick figures come from specialist clinics where neuropathic pain is common. FibriCheck's 95.6%/96.6% is versus ECG after dropping poor signals. Blood-culture 99.4%/99.7% is laboratory identification, not community screening. Frailty's 99.1% specificity describes a ~1.1% trajectory class, not a test you run on everyone.
- Supports · How well does CA125 find ovarian cancer in GP care?
- Supports · StEP pain-subtype assessment
- Supports · Smartphone PPG to detect atrial fibrillation
- Supports · Rising frailty flags one-year death risk
History
When this study was placed
Dated entries from the concept change log — when this paper was added or removed as support, challenge, or qualifier on a claim.
Removed as supporting evidence on Diagnostic Accuracy
Smartphone photoplethysmography detected atrial fibrillation with sensitivity about 95.6% and specificity about 96.6% versus ECG in an analysed clinic sample (adequate signal required; pacing excluded).
Placed as supporting evidence on Diagnostic Accuracy
A multiplex molecular assay on monomicrobial blood cultures identified Staphylococcus at genus level with sensitivity about 99.4% and specificity about 99.7% in a large evaluated set.
Removed as supporting evidence on Diagnostic Accuracy
Point-of-care classification accuracy (AF, blood culture ID, sickle cell, pain subtype) is not the same claim as low-prevalence screening PPV or long-horizon prognostic discrimination. Mixing sensitivities with hazards or PPVs invents a false head-to-head.
Related papers in this topic
Same topic cluster — not a recommendation engine.