Rapid gram-positive blood culture ID
A microarray panel identified Staphylococcus from positive blood cultures with ~99% sensitivity and specificity versus culture.
Source
Multiplex identification of gram-positive bacteria and resistance determinants directly from positive blood culture broths: evaluation of an automated microarray-based nucleic acid test
Study at a glance
- Design
- Other — Prospective diagnostic accuracy study of automated microarray panel vs reference culture
- N
- N=1157 · 1,157 prospectively collected monomicrobial positive blood cultures
- Population
- Monomicrobial positive blood cultures
- Outcome
- Sensitivity and specificity for gram-positive organisms and resistance markers
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Rapid gram-positive blood culture ID
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1,157 monomicrobial cultures.
A total of 1,157 prospectively collected blood cultures contained a single organism as determined by reference culture method. The
Staph sensitivity 99.4%.
Sensitivity of the Staphylococcus genus target was a combined 99.4% (840/845) (). Fiv
What they did
Prospective monomicrobial positive blood cultures were tested with an automated microarray nucleic-acid panel for gram-positive organisms and resistance markers against reference culture.
What they found
Among 1,157 monomicrobial cultures, Staphylococcus genus sensitivity was 99.4% and specificity 99.7%; staphylococci dominated the culture set.
The limits
What it doesn't show
Performance varies by organism/target; this evaluates assay accuracy, not downstream mortality benefit of faster reporting.
Key terms
- Blood culture broth
- Fluid from a positive culture bottle used for testing.
- Microarray nucleic-acid test
- Multiplex molecular panel for organisms/resistance genes.
- Sensitivity
- True-positive rate vs reference culture.
- Specificity
- True-negative rate vs reference culture.
- Monomicrobial
- Single organism recovered.
- Resistance determinant
- Gene marker linked to antibiotic resistance.
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Monomicrobial N:
Common questions
Monomicrobial N?
1,157.
Staph sensitivity?
99.4%.
Staph specificity?
99.7%.
Staph share?
73.0%.
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