cancer-biology
Do osteosarcoma cells stay diverse after metastasis?
Open access · cc by · source: Europe PMC
Across ~48k cells, osteosarcoma tumors kept distinct transcriptional subsets while adapting to bone and lung niches—even under clonal selection.
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Do osteosarcoma cells stay diverse after metastasis?
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47,977 cells profiled.
We investigated single-cell RNA-sequencing profiles of 47,977 cells obtained from cell line and patient-derived xenograft models
Tumor cells maintained phenotypic heterogeneity during bone/lung colonization.
Tumor cells maintained phenotypic heterogeneity as they responded to the selective pressures imposed during bone and lung colonization
Key findings
Heterogeneous transcriptional subsets persisted; a glucose-metabolism feature validated by immunofluorescence; lung colonization enriched multiple clones whose profiles stayed across generations.
Methodology
Profiled cell-line and PDX models with single-cell RNA-seq as tumors colonized tibia and lung, and combined lineage tracing with transcriptomics.
Limitations
Does not prove a single clinical therapy target or map early dissemination steps outside colonization bottlenecks.
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