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immunology

How do NK cells notice SARS-CoV-2–infected cells?

Hammer Q, Dunst J, Christ W, et al. · Cell reports · 2022

Open access · cc by · source: Europe PMC

SARS-CoV-2 Nsp13 encodes HLA-E–presented peptide VMPLSAPTL that stabilizes HLA-E but blocks NKG2A inhibition, favoring NKG2A+ NK attack.

Key findings

Nsp13 232–240 (VMPLSAPTL) was a top predicted binder. Unlike self-peptides, the viral peptide prevents HLA-E–NKG2A inhibitory binding, rendering targets susceptible to NK cells (missing-self). NKG2A-expressing NK cells are particularly activated in COVID-19 and can limit replication in vitro.

Methodology

Predicted HLA-E–binding SARS-CoV-2 nonamers, tested peptide stabilization of HLA-E, assessed NKG2A binding/inhibition, and linked findings to NKG2A+ NK activation in COVID-19 and in vitro control of infected lung epithelial cells.

Limitations

Does not prove this pathway alone determines COVID-19 severity in patients, or that boosting NKG2A+ NK cells is a safe therapy.

How this study connects

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