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neuroimmunology

Does vagal TLR4 cause LPS anorexia—or mainly CGRP release?

Jia L, Lee S, Tierney JA, et al. · eNeuro · 2021

Open access · cc by · source: Europe PMC

Restoring TLR4 only in Nav1.8 afferents did not restore LPS anorexia, but LPS still triggered CGRP release from TLR4+/CGRP+ vagal jugular neurons.

Key findings

Nav1.8-restricted TLR4 was insufficient for rapid-onset LPS anorexia; Tlr4 enriched in jugular Nav1.8/CGRP vagal afferents; LPS stimulated CGRP release in vitro.

Methodology

Built Tlr4 LoxTB mice, restored TLR4 globally (Zp3-Cre) or in Nav1.8 afferents, mapped Tlr4 with ISH, and applied LPS to cultured afferents.

Limitations

Does not map the full in vivo infection-fighting role of the LPS–TLR4–CGRP loop or test female experimental cohorts for feeding assays.

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