Cell signalling
How specific are bacterial two-component pathways?
Open access · cc by · source: Europe PMC
Caulobacter histidine kinases prefer their cognate response regulators in vitro; phosphotransfer profiling reveals conserved pathways controlling growth.
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How specific are bacterial two-component pathways?
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Do Caulobacter histidine kinases prefer cognate regulators?
C. crescentus histidine kinases have an in vitro kinetic preference for their in vivo cognate substrate.
What method rapidly maps kinase targets?
phosphotransfer profiling, to rapidly identify the targets of histidine kinases ().
Key findings
Kinases show kinetic preference for in vivo cognate regulators; profiling identified targets such as CC0530→CC3743 and the conserved CenK–CenR envelope/growth pathway.
Methodology
Combined genetics and phosphotransfer profiling across Caulobacter two-component proteins to map kinase–regulator pairs and phenotypes.
Limitations
Does not claim every environmental input for every kinase in nature is solved.
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