What cell programs mark ischemic cardiomyopathy?
snRNA-seq of ~100k cardiac nuclei showed fewer cardiomyocytes and more specialized endothelial states in ICM, overlapping other end-stage cardiomyopathy programs.
Source
Single-nucleus RNA sequencing in ischemic cardiomyopathy reveals common transcriptional profile underlying end-stage heart failure
Study at a glance
- Design
- Case-control — snRNA-seq of non-infarct LV from ICM transplant recipients vs NF controls
- N
- N=15 · 7 ICM + 8 NF; 99,684 nuclei after QC
- Population
- Human LV nuclei from ICM and non-failing hearts
- Outcome
- Cell-composition and transcriptional differences in ICM vs NF
Structured fields used in claim comparison tables when every cited study has a complete layer.
What they did
Profiled non-infarct LV nuclei from ICM transplant recipients and non-failing controls with snRNA-seq.
What they found
99,684 QC nuclei in 16 clusters. ICM had fewer cardiomyocytes and more lymphatic/angiogenic/arterial endothelial cells; transcriptional changes resembled HCM/DCM datasets.
The limits
What it doesn't show
End-stage transplant tissue may not represent early ICM; composition shifts are associative.
Key terms
- snRNA-seq
- Single-nucleus RNA sequencing.
- Ischemic cardiomyopathy
- Heart failure after ischemic injury/remodeling.
- Non-failing control
- Comparison hearts without end-stage failure.
- Endothelial subtypes
- Lymphatic, angiogenic, arterial EC states.
- LAMININ signaling
- ECM pathway highlighted from endothelium.
- Druggable genes
- Shared cardiomyopathy targets suggested by dataset mining.
Flashcards
Research intelligence for this paper
See its role on concept claims, tensions it is part of, placement history, and related discoveries.
Quiz yourself
QC nuclei ~
Common questions
Nuclei after QC?
99,684.
Donors?
7 ICM + 8 NF.
Composition shift?
Fewer cardiomyocytes; more specialized ECs.
Cross-disease?
Similar to HCM/DCM programs.
More on Gene expression