Cataloging mammalian circular RNAs
Thousands of mammalian circRNAs arise by back-splicing and can be quantified across ENCODE cell types.
Source
Expanded identification and characterization of mammalian circular RNAs
What they did
Built a computational pipeline on non-poly(A) RNA-seq to detect back-splice junctions and circular fractions.
What they found
Most confident circRNAs use canonical GT-AG splice signals; circular fractions vary by locus and cell type.
The limits
What it doesn't show
Does not prove sponge function for most newly found circRNAs.
Key terms
- circRNA
- Covalently circular RNA isoform.
- Back-splicing
- Donor joins upstream acceptor.
- miRNA sponge
- RNA that titrates miRNAs.
- ENCODE
- Large functional genomics consortium.
- Circular fraction
- Share of circular vs linear isoform.
- GT-AG
- Canonical splice-site dinucleotides.
Flashcards
Want these cards to stick?
Save the deck to NoteFren and study it with spaced repetition.
Quiz yourself
Topic:
Common questions
Biogenesis?
Back-splicing.
Data?
ENCODE RNA-seq.
Splice signals?
Mostly GT-AG.
Example sponge?
CDR1as.
More on Gene expression