Cataloging mammalian circular RNAs

Open paper intelligence

Thousands of mammalian circRNAs arise by back-splicing and can be quantified across ENCODE cell types.

Source

Expanded identification and characterization of mammalian circular RNAs

Guo JU, Agarwal V, Guo H, et al. · Genome biology · 2014

doi.org/10.1186/s13059-014-0409-z Read the full paper ↗1,316 citationscc by

Study at a glance

Design: Computational / modelling — Computational detection of back-splice junctions in non-poly(A) RNA-seq
N: Multi-sample circRNA discovery pipeline — no single primary analytic N in stored summary
Population: Mammalian non-poly(A) RNA-seq libraries
Outcome: Expanded catalogue and splice features of circular RNAs

Structured fields used in claim comparison tables when every cited study has a complete layer.

Inspect source passages

Cataloging mammalian circular RNAs

These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.

Mammalian circular RNAs.

Background Many classes of non-protein-coding RNAs (ncRNAs) exist in cells [,], and members of each class play important roles in either regulating gene expression or other biological processes [–].

Back-splicing of pre-mRNA.

For example, microRNAs (miRNAs) pair to sites within messenger RNAs (mRNAs) to target the mRNAs for translational repression and/or mRNA destabilization [].

Read passages in the original paper

What they did

Built a computational pipeline on non-poly(A) RNA-seq to detect back-splice junctions and circular fractions.

What they found

Most confident circRNAs use canonical GT-AG splice signals; circular fractions vary by locus and cell type.

The limits

What it doesn't show

Does not prove sponge function for most newly found circRNAs.

Key terms

circRNA: Covalently circular RNA isoform.
Back-splicing: Donor joins upstream acceptor.
miRNA sponge: RNA that titrates miRNAs.
ENCODE: Large functional genomics consortium.
Circular fraction: Share of circular vs linear isoform.
GT-AG: Canonical splice-site dinucleotides.

Flashcards

1 / 10

0 of 10 answers reviewed

Research intelligence for this paper

See its role on concept claims, tensions it is part of, placement history, and related discoveries.

Open paper intelligence

Quiz yourself

1 / 6

Topic:

Common questions

Biogenesis?

Back-splicing.

Data?

ENCODE RNA-seq.

Splice signals?

Mostly GT-AG.

Example sponge?

CDR1as.