Host–pathogen
Pathogenicity factors in Naegleria fowleri
Open access · cc by · source: Europe PMC
Whole-genome analysis of N. fowleri highlights candidate pathogenicity factors for brain infection.
How this study connects
Role on claims
Each row is a claim on a concept or method page where this paper supports, challenges, or qualifies the statement. Roles are hand-checked — not a model guess.
Three papers are false-positive lexicon hits for proteomics. Naegleria fowleri work assembled an ~30 Mb AT-rich genome and listed candidate pathogenicity genes; Aspergillus comparative genomics reported ~29–36 Mb genomes with 9,113–13,553 genes (~20% Aspergillaceae-specific; section Nigri ~1,800 unique genes); Plasmodium falciparum IDC transcriptome was hourly expression across a ~48-hour blood-stage cycle. Those are DNA or RNA catalogues, not peptide spectra.
Evidence for the claim as stated.
Naegleria and Aspergillus genomes and the Plasmodium IDC transcriptome should not be cited as mass-spectrometry evidence at all. Candidate pathogenicity gene lists and periodic RNA profiles are sequence resources that still need protein-level or infection-model tests.
Evidence for the claim as stated.
Two papers barely use flow cytometry as the core method. Spike glycosylation/palmitoylation trafficking used LYQD and cysteine-cluster mutants plus 2-bromopalmitate, scoring S2 mobility, packaging and ACE2 fusion — cell-based trafficking, not a leukocyte panel. Naegleria fowleri work assembled an ~30 Mb AT-rich genome and listed pathogenicity candidates that still need infection-model tests.
Evidence for the claim as stated.
Foxp3+ gating is a means to an epigenetic comparison (TSDR demethylation versus unstable induced Foxp3), whereas spike and Naegleria papers are trafficking genetics and a genome assembly. Treating every hit as a flow-cytometry methods paper hides those mismatches.
Evidence for the claim as stated.
Open questions
Tensions this paper is part of
From concept pages' “where studies disagree.” Disagreement means the same question; scope means different assays, populations, or outcomes.
Naegleria and Aspergillus genomes and the Plasmodium IDC transcriptome should not be cited as mass-spectrometry evidence at all. Candidate pathogenicity gene lists and periodic RNA profiles are sequence resources that still need protein-level or infection-model tests.
- Supports · Aspergillus genomic diversity
- Supports · How does malaria’s blood-stage transcriptome unfold?
Foxp3+ gating is a means to an epigenetic comparison (TSDR demethylation versus unstable induced Foxp3), whereas spike and Naegleria papers are trafficking genetics and a genome assembly. Treating every hit as a flow-cytometry methods paper hides those mismatches.
- Supports · Epigenetic locking of Foxp3 in Tregs
- Supports · Spike glycosylation and palmitoylation trafficking
Study at a glance
- Design
- Computational / modelling — De novo genome sequencing plus proteomics to nominate N. fowleri pathogenicity factors
- N
- ~29.6 Mb assembled nuclear genome (diploid estimate ~66 Mb); >500 million reads — genome resource study
- Population
- Naegleria fowleri (compared with non-pathogenic N. gruberi)
- Outcome
- Candidate pathogenicity gene sets from genome/proteome comparison
Structured fields used in claim comparison tables when every cited study has a complete layer.
Inspect source passages
Pathogenicity factors in Naegleria fowleri
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
Naegleria fowleri.
Although approximately 30 species have been recognized so far, Naegleria fowleri is the only human pathogen that causes primary amoebic meningoencephalitis (PAM) []. Infection occurs when water contaminated by N.
Primary amoebic meningoencephalitis.
However, no more than a dozen patients out of approximately 350 reported PAM cases have been treated successfully with amphotericin B, either alone or in combination with other drugs [–]. Hence, N.
Key findings
~30 Mb AT-rich genome assembled; candidate pathogenicity gene sets identified for follow-up.
Methodology
Sequenced and assembled the N. fowleri genome and compared features relevant to virulence.
Limitations
Genome candidates need functional validation in infection models.
Related papers in this topic
Same topic cluster — not a recommendation engine.