Host–pathogen
How does malaria’s blood-stage transcriptome unfold?
Open access · cc by · source: Europe PMC
Plasmodium falciparum’s 48-hour intraerythrocytic cycle was profiled hourly, revealing tightly staged gene-expression programs after RBC invasion.
Study at a glance
- Design
- Animal / in-vitro — Hourly IDC transcriptome of P. falciparum across the ~48-hour erythrocytic cycle
- N
- Time-series parasite culture transcriptome — no single sample N
- Population
- Plasmodium falciparum during the intraerythrocytic developmental cycle
- Outcome
- Stage-ordered periodic gene expression across the IDC
Structured fields used in claim comparison tables when every cited study has a complete layer.
Key findings
Gene expression is highly periodic and stage-ordered across the IDC starting with merozoite invasion and parasitophorous vacuole formation.
Methodology
Measured the IDC transcriptome of P. falciparum at 1-hour resolution across the ~48-hour cycle from merozoite invasion through ring/trophozoite/schizont stages.
Limitations
Does not itself deliver a drug; focuses on blood-stage expression timing.
How this study connects
Role on claims
Each row is a claim on a concept or method page where this paper supports, challenges, or qualifies the statement. Roles are hand-checked — not a model guess.
Multiple empirical papers in this library examine host–pathogen with mechanistic biological findings.
Evidence for the claim as stated.
Gene expression is highly periodic and stage-ordered across the IDC starting with merozoite invasion and parasitophorous vacuole formation.
Evidence for the claim as stated.
Systems and scales differ across host–pathogen studies (species, tissues, methods), so mechanisms should not be over-generalised.
Evidence for the claim as stated.
Three papers are false-positive lexicon hits for proteomics. Naegleria fowleri work assembled an ~30 Mb AT-rich genome and listed candidate pathogenicity genes; Aspergillus comparative genomics reported ~29–36 Mb genomes with 9,113–13,553 genes (~20% Aspergillaceae-specific; section Nigri ~1,800 unique genes); Plasmodium falciparum IDC transcriptome was hourly expression across a ~48-hour blood-stage cycle. Those are DNA or RNA catalogues, not peptide spectra.
Evidence for the claim as stated.
Naegleria and Aspergillus genomes and the Plasmodium IDC transcriptome should not be cited as mass-spectrometry evidence at all. Candidate pathogenicity gene lists and periodic RNA profiles are sequence resources that still need protein-level or infection-model tests.
Evidence for the claim as stated.
Open questions
Tensions this paper is part of
From concept pages' “where studies disagree.” Disagreement means the same question; scope means different assays, populations, or outcomes.
Systems and scales differ across host–pathogen studies (species, tissues, methods), so mechanisms should not be over-generalised.
- Supports · LN DCs shelter antibiotic-tolerant Salmonella
- Supports · PD-1 blockade reactivates TB via TNF
Naegleria and Aspergillus genomes and the Plasmodium IDC transcriptome should not be cited as mass-spectrometry evidence at all. Candidate pathogenicity gene lists and periodic RNA profiles are sequence resources that still need protein-level or infection-model tests.
- Supports · Pathogenicity factors in Naegleria fowleri
- Supports · Aspergillus genomic diversity
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