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Nitrogen cycle

Vanadium nitrogenase fuels forest moss N fixation

Darnajoux R, Haynes SJ, Renaudin M, et al. · Environmental science & technology · 2026

Open access · cc by · source: Europe PMC

Vanadium nitrogenase was active in most valid moss and lichen samples across three continents, averaging about 40% of moss N2 fixation.

Study at a glance

Design
Cross-sectional — LISARA assays of moss and lichen V-nitrogenase across three continents
N
N=109 · 109 sampling plots (>40 sites, 9 locations); multiple cryptogam samples per plot
Population
Mosses and lichens in extratropical forests of North America, South America, and East Asia
Outcome
Share of biological N2 fixation attributable to vanadium nitrogenase

Structured fields used in claim comparison tables when every cited study has a complete layer.

Key findings

V-Nase occurred in 68±20% of samples by site (79% of valid assays), 15 of 28 genera, from 28% of Hokkaido samples to 94% in North Carolina; mosses ~40% and lichens ~25% of BNF.

Methodology

Used low-activity isotopic acetylene reduction (LISARA) on cryptogams from over 40 sites in 9 locations across North America, South America, and East Asia.

Limitations

Isotopes cannot yet separate V-only from Fe-only nitrogenase, and assays do not scale measured rates to a global N budget.

How this study connects

Role on claims

Each row is a claim on a concept or method page where this paper supports, challenges, or qualifies the statement. Roles are hand-checked — not a model guess.

  • SupportsNitrogen cycleconcept

    This library holds 6 empirical papers on nitrogen cycle with measured outcomes rather than reviews.

    Evidence for the claim as stated.

  • SupportsNitrogen cycleconcept

    Vanadium nitrogenase was active in most valid moss and lichen samples across three continents, averaging about 40% of moss N2 fixation.

    Evidence for the claim as stated.

  • SupportsNitrogen cycleconcept

    A reactor microbiome or a canopy 15N tracer is not catchment-scale load.

    Evidence for the claim as stated.

Open questions

Tensions this paper is part of

From concept pages' “where studies disagree.” Disagreement means the same question; scope means different assays, populations, or outcomes.

Related papers in this topic

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