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Habitat restoration

Cellulose plus C4 grass steers prairie soil microbes

Docherty KM, Gutknecht JLM · Ecological applications : a publication of the Ecological Society of America · 2019

Open access · cc by · source: Europe PMC

Adding cellulose and heat-tolerant Andropogon gerardii shifted restored prairie soils toward higher fungal biomass and slower-growing bacteria that may retain carbon.

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Cellulose plus C4 grass steers prairie soil microbes

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DNA was extracted from each of 72 treated pots.

we defrosted frozen soils collected from each of the 72 treated pots and extracted total genomic DNA using a PowerSoil DNA Isolation Kit

Warming room was +4°C above ambient.

One room was kept at ambient temperature while we elevated the temperature in the other room to + 4°C above ambient.
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Key findings

Cellulose raised fungal:bacterial lipid ratios (18:2ω6,9c and 18:1ω9c up; Gram-positive lipids down) regardless of plant mix. Plant richness had few microbial effects. Coupling cellulose with heat/drought-tolerant grasses selected higher fungal biomass and oligotroph-leaning bacteria.

Methodology

Agricultural soil in 72 pots received remnant-prairie inoculum and/or cellulose, planted with one vs three prairie species, then grown 62 days at ambient or +4°C; lipids, enzymes, 16S, and soil C were measured.

Limitations

A 62-day pot study cannot prove long-term field carbon sequestration; watering prevented drought so warming×moisture interactions were muted.

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