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Three CpGs track blood aging

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Age-related blood DNA methylation at a few CpGs predicts chronological age with ~3.3-year MAD.

Source

Aging of blood can be tracked by DNA methylation changes at just three CpG sites

Weidner CI, Lin Q, Koch CM, et al. · Genome biology · 2014

doi.org/10.1186/gb-2014-15-2-r24Read the full paper ↗687 citationscc by

Study at a glance

Design
Computational / modelling — Combined blood DNAm datasets to select age-related CpGs and train a 3-site age predictor
N
N=575 · 575 DNAm profiles from four studies, ages 0–78
Population
Human blood DNA methylation profiles across childhood to older adulthood
Outcome
Epigenetic age prediction from blood CpG methylation

Structured fields used in claim comparison tables when every cited study has a complete layer.

What they did

Combined 575 blood DNAm profiles (ages 0–78), selected age-correlated CpGs, and trained predictors including a 3-site bisulfite assay.

What they found

102 AR-CpGs (58 hypo, 44 hyper); a three-CpG signature predicts age with MAD 3.34 years (R²=0.98).

The limits

What it doesn't show

Does not prove CpG changes cause aging diseases.

Key terms

DNAm
DNA methylation at CpG cytosines.
AR-CpG
Age-related CpG site.
Epigenetic clock
Methylation-based age predictor.
MAD
Mean absolute deviation of predicted vs true age.
Bisulfite sequencing
Assay converting unmethylated C→T.
Hypermethylation
Age-linked gain of methylation at some CpGs.

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Profiles n:

Common questions

How many profiles?

575.

AR-CpGs selected?

102.

Minimal signature?

Three CpG sites.

MAD?

3.34 years.

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