What do plant respiratory supercomplexes look like?
Cryo-EM of Vigna radiata CIII2/CIV/SC III2+IV revealed plant-specific MPP-domain features and coordinated CIII2 motions.
Source
Atomic structures of respiratory complex III<sub>2</sub>, complex IV, and supercomplex III<sub>2</sub>-IV from vascular plants
Study at a glance
- Design
- Animal / in-vitro — Single-particle cryo-EM of plant mitochondrial CIII2, CIV, SC III2+IV
- N
- N=3 · Three assemblies modeled from Vigna radiata
- Population
- Vigna radiata mitochondrial respiratory complexes
- Outcome
- Atomic structures and CIII2 conformational dynamics
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Inspect source passages
What do plant respiratory supercomplexes look like?
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
Atomic models of three assemblies.
We present atomic models of CIII 2 , CIV, and SC III 2 +IV from Vigna radiata determined by single-particle cryoEM.
CIII2 and CIV form SC III2+IV.
Abstract Mitochondrial complex III (CIII 2 ) and complex IV (CIV), which can associate into a higher-order supercomplex (SC III 2 +IV), play key roles in respiration.
What they did
Determined single-particle cryo-EM structures of mitochondrial CIII2, CIV, and their supercomplex from mung bean.
What they found
Plant-specific MPP-domain differences, defined CIV subunits, and long-range coordinated CIII2 movements including the iron-sulfur head domain.
The limits
What it doesn't show
Plant maps may differ from mammalian complexes; static ensembles undersample full catalysis.
Key terms
- Complex III2
- Dimeric cytochrome bc1 complex.
- Complex IV
- Cytochrome c oxidase.
- Supercomplex III2+IV
- Higher-order CIII2+CIV assembly.
- Cryo-EM
- Cryogenic electron microscopy.
- MPP domain
- Mitochondrial processing peptidase-related CIII2 region.
- Iron-sulfur head domain
- Mobile Rieske domain of CIII2.
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Quiz yourself
Assemblies:
Common questions
Organism?
Vigna radiata.
Assemblies?
CIII2, CIV, SC III2+IV.
Method?
Single-particle cryo-EM.
Dynamics?
Coordinated long-range CIII2 motions.
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