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Blackberry-leaf silver nanoparticles against plant pathogens

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Rubus fruticosus extract reduces Ag+ to 50–120 nm AgNPs (SPR 449 nm) that fully inhibit two phytopathogens at the highest dose.

Source

Biogenic synthesis of silver nanoparticles using <i>Rubus fruticosus</i> extract and their antibacterial efficacy against <i>Erwinia caratovora</i> and <i>Ralstonia solanacearum</i> phytopathogens

Khan A, Ahmad N, Fazal H, et al. · RSC advances · 2024

doi.org/10.1039/d3ra06723hRead the full paper ↗7 citationscc by

Study at a glance

Design
Animal / in-vitro — Plant-extract AgNP synthesis with antibacterial titration against plant pathogens and DPPH assays
N
Materials/antimicrobial assay study — no sample N
Population
Ralstonia solanacearum and Erwinia carotovora cultures exposed to Rubus-derived AgNPs
Outcome
Pathogen inhibition and antioxidant activity of biogenic AgNPs

Structured fields used in claim comparison tables when every cited study has a complete layer.

What they did

Authors mixed leaf extract with AgNO3, confirmed SPR, XRD, TEM and EDX, then titrated nanoparticles versus Ralstonia solanacearum and Erwinia carotovora, plus DPPH antioxidant assays.

What they found

SPR at 449 nm (stable 4 months). XRD peaks at 38.1°, 44.4°, 54.5°, 77.9°. TEM 50–120 nm. EDX ~90% Ag at 3 keV. Highest NP dose gave 100% inhibition of both pathogens; lower doses still beat extract alone.

The limits

What it doesn't show

Field efficacy, mammalian toxicity, and a fully assigned capping-molecule structure are not established; ‘T1–T10’ doses need the paper’s table to translate to µg mL−1.

Key terms

Biogenic AgNPs
Silver nanoparticles reduced/stabilized by plant phytochemicals instead of NaBH4.
SPR
Surface plasmon resonance band diagnostic of Ag(0) colloids (~400–480 nm).
Ralstonia / Erwinia
Bacterial phytopathogens used as antibacterial test organisms.
EDX 3 keV peak
Elemental silver signature used to estimate ~90% Ag.
DPPH assay
517 nm radical-scavenging test of antioxidant capacity.

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Diagnostic SPR of these AgNPs:

Common questions

What SPR wavelength?

449 nm.

TEM size?

50–120 nm.

Highest NP dose vs pathogens?

100% inhibition.

EDX Ag fraction?

About 90%.

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