Gamma-made Ag–Se nanoparticles carrying cefotaxime
Radiolytic Ag, Se, and Ag–Se nanoparticles (about 11–21 nm) are characterized and loaded with cefotaxime for synergistic antimicrobial tests.
Source
Cefotaxime incorporated bimetallic silver-selenium nanoparticles: promising antimicrobial synergism, antibiofilm activity, and bacterial membrane leakage reaction mechanism
Study at a glance
- Design
- Animal / in-vitro — Radiolytic synthesis of Ag, Se, and Ag–Se nanoparticles loaded with cefotaxime for antimicrobial/antibiofilm assays
- N
- Particle characterization and multi-organism assays rather than a single sample N
- Population
- Bacterial cultures in antimicrobial and antibiofilm tests
- Outcome
- Cefotaxime-loaded Ag–Se nanoparticles show synergistic antimicrobial and antibiofilm activity with membrane-leakage evidence
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Inspect source passages
Gamma-made Ag–Se nanoparticles carrying cefotaxime
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
Average sizes are 10.95, 20.54, and 12.69 nm for Ag, Se, and Ag–Se NPs.
Complete characterization is performed to analyze the shape, crystallinity, distribution, and size of the synthesized NPs, which are shown to be spherical with an average size of 10.95, 20.54, and 12.69 nm for Ag NPs, Se NPs, and Ag–Se NPs, respectively.
Gamma doses were 15.0 kGy for Ag and Ag–Se NPs and 10 kGy for Se NPs.
A suggested reaction mechanism describing the potential and constant reduction of ions due to the reduction by 15.0 kGy gamma rays (for Ag NPs, and Ag–Se NPs) and 10 kGy (for Se NPs) is introduced.
What they did
Authors reduced 1.0 mM AgNO3 and/or Na2SeO3 with gamma rays (15 kGy Ag/Ag–Se; 10 kGy Se), tracked UV-vis (406/518/420 nm), sized particles by DLS/HR-TEM/XRD, and combined them with cefotaxime for antimicrobial/antibiofilm assays.
What they found
Spherical particles averaging 10.95 nm (Ag), 20.54 nm (Se), and 12.69 nm (Ag–Se). Optical densities were recorded at 406, 518, and 420 nm. A radiolytic reduction mechanism at those kGy doses is proposed.
The limits
What it doesn't show
This is not a randomized clinical UTI trial; membrane-leakage/MIC biology is secondary to the materials characterization for chemistry teaching.
Key terms
- Gamma radiolysis
- Ionizing radiation that generates reducing radicals to make metal nanoparticles.
- Bimetallic Ag–Se NPs
- Mixed silver–selenium nanoparticles from co-reduction of Ag+ and SeO32−.
- Cefotaxime (CFM)
- β-lactam antibiotic incorporated with the NPs.
- SPR wavelengths
- 406 nm Ag, 518 nm Se, 420 nm Ag–Se used to track formation.
- kGy dose
- 15 kGy for Ag/Ag–Se and 10 kGy for Se NPs.
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Average Ag NP size is about:
Common questions
Average TEM sizes?
10.95, 20.54, and 12.69 nm for Ag, Se, Ag–Se.
Radiation doses?
15 kGy (Ag, Ag–Se) and 10 kGy (Se).
UV-vis wavelengths?
406, 518, 420 nm.
Precursor concentration?
1.0 mM salts.
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