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Shared class A GPCR activation path

Class A GPCRs share a conserved residue-contact rearrangement pathway from ligand pocket to G-protein site.

Source

Common activation mechanism of class A GPCRs

Zhou Q, Yang D, Wu M, et al. · eLife · 2019

doi.org/10.7554/elife.50279Read the full paper ↗560 citationscc by

What they did

Quantified residue–residue contact scores across inactive/active structures and identified conserved switching/repacking contacts.

What they found

A 34-residue-pair pathway across layers; comparisons used 142 inactive and 27 active structures (p<0.001).

The limits

What it doesn't show

Does not replace ligand-specific pharmacology for every receptor.

Key terms

GPCR
G protein–coupled receptor.
Class A
Largest rhodopsin-like GPCR family.
RRCS
Residue–residue contact score.
Transmission switch
Conserved contact set linking pocket to core.
Microswitch
Local motif rewiring during activation.
Active vs inactive
Conformational states relative to G-protein coupling.

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Quiz yourself

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Residue pairs:

Common questions

Pathway size?

34 residue pairs (35 residues).

Inactive structures?

142.

Active structures?

27.

First common step?

Transmission switch + Na⁺ pocket changes.

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