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How did some Enterococcus faecium become hospital pathogens?

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Comparative pangenomes split E. faecium into a mixed-source subclade I and a human-only subclade II with larger genomes packed with resistance, virulence, bacteriocin, and mobile genes likely gained by HGT.

Source

Comparative pangenome analysis of Enterococcus faecium and Enterococcus lactis provides new insights into the adaptive evolution by horizontal gene acquisitions

Choi DG, Baek JH, Han DM, et al. · BMC genomics · 2024

doi.org/10.1186/s12864-023-09945-7Read the full paper ↗29 citationscc by

Study at a glance

Design
Computational / modelling — Comparative pangenome analysis of dereplicated E. faecium and E. lactis genomes
N
All available dereplicated genomes of the two species (counts in full text tables)
Population
Enterococcus faecium and Enterococcus lactis genomes from diverse isolation sources
Outcome
Subclade structure and enrichment of resistance/virulence/mobile genes

Structured fields used in claim comparison tables when every cited study has a complete layer.

What they did

They analyzed all available dereplicated genomes of E. faecium and closely related E. lactis, comparing phylogeny, genome size/gene content, and resistance/virulence gene histories.

What they found

Subclade II was exclusively human-derived, larger, and richer in resistance/virulence/mobile elements; phylogenetic patterns support horizontal acquisition tied to antibiotic pressure. E. lactis did not cluster by isolation source.

The limits

What it doesn't show

Genome catalogs do not measure in-host virulence experimentally or prove causality for every gene’s clinical effect.

Key terms

Pangenome
Union of genes across all genomes of a species.
E. faecium
Enterococcus species that can be commensal or nosocomial pathogen.
E. lactis
Closely related Enterococcus often linked to foods/commensal niches.
Subclade II
More recent human-restricted E. faecium lineage in this study.
Horizontal gene transfer
Gene acquisition between strains other than vertical inheritance.
Mobile element
DNA that can move within/between genomes (e.g., plasmids, IS).

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Subclade II isolation sources:

Common questions

How many E. faecium subclades?

Two: mixed-source I and human-only II.

What is special about subclade II?

Larger genomes; more resistance/virulence/bacteriocin/mobile genes.

Proposed acquisition route?

Horizontal gene transfer under antibiotic pressure.

E. lactis clustering?

No clear clustering by isolation source.

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