Selective bacterial BSH shifts host metabolism
Bacteroides bile salt hydrolase BT2086 selectively deconjugates bile acids and alters host metabolism.
Source
A selective gut bacterial bile salt hydrolase alters host metabolism
Study at a glance
- Design
- Animal / in-vitro — Bacteroidetes BSH selectivity screen plus B. thetaiotaomicron BT2086 deletion in gnotobiotic mice
- N
- ~20 Bacteroidetes strains screened; monocolonization used 12 mice per Bt WT/KO group (8 GF controls) — no single primary N
- Population
- Human-gut Bacteroidetes strains and germ-free C57BL/6 mice
- Outcome
- Host metabolic phenotypes after selective bile-salt hydrolase loss
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Selective bacterial BSH shifts host metabolism
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
Twenty Bacteroidetes strains screened.
To investigate this question, we performed a screen of the BSH activity of twenty Bacteroidetes strains found in the human gut ( and and ) ().
Hydrolysis steady state by 48 hr.
We monitored deconjugation over time by UPLC-MS and determined that all hydrolysis reactions had reached steady state by 48 hr (, ).
What they did
Screened Bacteroidetes BSH selectivity, deleted BT2086 in B. thetaiotaomicron, and colonized mice to read host metabolic phenotypes.
What they found
Among ~20 Bacteroidetes strains, some BSHs prefer steroid cores; BtΔ2086 loses deconjugation; colonization changes weight on high-fat diet vs WT.
The limits
What it doesn't show
Human intervention efficacy; one enzyme among many microbiome functions.
Key terms
- BSH
- Bile salt hydrolase deconjugating bile acids.
- BT2086
- B. thetaiotaomicron BSH gene/enzyme.
- Deconjugation
- Removal of glycine/taurine from bile acids.
- Gnotobiotic
- Host with defined/known microbes.
- Bacteroides
- Common Gram-negative gut genus.
- Bile acids
- Host steroids modified by gut bacteria.
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Key gene:
Common questions
Key gene?
BT2086.
Strains screened?
About 20 Bacteroidetes.
KO phenotype?
Lost conjugated bile-acid cleavage.
Host readout?
Metabolism/weight on high-fat diet.
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