Aloe vera medicinal pathway transcriptome
De novo root and leaf transcriptomes highlight saponin and anthraquinone metabolism genes.
Source
De novo sequencing, assembly and characterisation of Aloe vera transcriptome and analysis of expression profiles of genes related to saponin and anthraquinone metabolism
What they did
Illumina RNA-seq of Aloe vera root and leaf with Trinity assembly and pathway annotation.
What they found
~43k CDS per tissue with extensive annotation tied to secondary-metabolite pathways.
The limits
What it doesn't show
Does not chemically quantify every metabolite or prove each enzyme activity.
Key terms
- De novo transcriptome
- Assembly without a reference genome.
- CDS
- Predicted coding sequence.
- Saponin
- Glycoside secondary metabolite.
- Anthraquinone
- Aromatic Aloe metabolite.
- Unigene
- Clustered non-redundant transcript.
- Secondary metabolism
- Specialized plant biosynthetic pathways.
Flashcards
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Topic:
Common questions
Tissues?
Root and leaf.
Assembler?
Trinity.
Focus?
Saponin/anthraquinone.
Organism?
Aloe vera.
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