Aloe vera medicinal pathway transcriptome

De novo root and leaf transcriptomes highlight saponin and anthraquinone metabolism genes.

Source

De novo sequencing, assembly and characterisation of Aloe vera transcriptome and analysis of expression profiles of genes related to saponin and anthraquinone metabolism

Choudhri P, Rani M, Sangwan RS, et al. · BMC genomics · 2018

doi.org/10.1186/s12864-018-4819-2 Read the full paper ↗33 citationscc by

Study at a glance

Design: Computational / modelling — Illumina RNA-seq de novo Trinity assembly of Aloe vera root and leaf transcriptomes
N: Two tissues; ~43,443 root and ~43,178 leaf CDS — transcriptome resource, not a sample-N study
Population: Aloe vera root and leaf tissues
Outcome: Annotated transcriptome resources linked to secondary-metabolite pathways

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Aloe vera medicinal pathway transcriptome

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Aloe vera.

The genus contains more than 360 species, out of which only four have been reported to exhibit medicinal properties: Aloe vera, Aloe arborescens, Aloe ferox, and Aloe perryi.

Root and leaf.

Many of the medicinal properties of Aloe vera are ascribed to secondary metabolites though they are relatively minor in their concentration in the plant (< 1% dry weight) [].

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What they did

Illumina RNA-seq of Aloe vera root and leaf with Trinity assembly and pathway annotation.

What they found

~43k CDS per tissue with extensive annotation tied to secondary-metabolite pathways.

The limits

What it doesn't show

Does not chemically quantify every metabolite or prove each enzyme activity.

Key terms

De novo transcriptome: Assembly without a reference genome.
CDS: Predicted coding sequence.
Saponin: Glycoside secondary metabolite.
Anthraquinone: Aromatic Aloe metabolite.
Unigene: Clustered non-redundant transcript.
Secondary metabolism: Specialized plant biosynthetic pathways.

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Common questions

Tissues?

Root and leaf.

Assembler?

Trinity.

Focus?

Saponin/anthraquinone.

Organism?

Aloe vera.