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Intermittent aeration and anammox nitrogen

Open paper intelligence

Switching oxygen on and off kept anammox working while wiping out nitrite-oxidizing bacteria.

Source

Efficient management of the nitritation-anammox microbiome through intermittent aeration: absence of the NOB guild and expansion and diversity of the NOx reducing guild suggests a highly reticulated nitrogen cycle

Palomo A, Azevedo D, Touceda-Suárez M, et al. · Environmental microbiome · 2022

doi.org/10.1186/s40793-022-00432-2Read the full paper ↗3 citationscc by

What they did

Operated a partial nitritation–anammox granule reactor with rising redox-switch frequency and recovered 55 MAGs from monthly metagenomes.

What they found

TN removal stayed about 75–86%; NOB were essentially absent; AOB ~13% and anammox ~22% of the community; 79% of reads mapped to 55 MAGs.

The limits

What it doesn't show

Does not demonstrate the same NOB suppression in full-scale plants with real wastewater organics.

Key terms

Anammox (AnAOB)
Anaerobic ammonium-oxidizing bacteria.
AOB
Aerobic ammonia-oxidizing bacteria.
NOB
Nitrite-oxidizing bacteria that make nitrate.
PNA
Partial nitritation–anammox nitrogen removal.
MAG
Metagenome-assembled genome.
Intermittent aeration
Cycling oxygen on and off to steer the microbiome.

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MAGs recovered:

Common questions

How many MAGs?

55.

Early TN removal?

About 86 ± 4%.

Anammox share?

About 22% of the metagenome.

NOB outcome?

Canonical NOB essentially eliminated.

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