Can LAB exopolysaccharides act as therapeutic biomolecules?
Among 10 strains, L. delbrueckii EPS5 had top yield and strong cytotoxicity (IC50 ~7.9–10.7 mg/ml) plus fibroblast-supportive effects.
Source
Exploring the Therapeutic Potentials of Exopolysaccharides Derived From Lactic Acid Bacteria and Bifidobacteria: Antioxidant, Antitumor, and Periodontal Regeneration
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Can LAB exopolysaccharides act as therapeutic biomolecules?
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EPS yields ranged 174.9–295.4 mg/l.
The EPS derived from bacteria ranged from 174.9 to 295.4 mg/l
EPS5 yield 295.4 ± 9.7 mg/l (highest).
L. delbrueckii ssp. bulgaricus DSM 20081 T produced the most EPS (EPS 5 , 295.4 ± 9.7)
What they did
Isolated EPS from LAB/Bb supernatants and tested concentration-dependent antioxidant, MTT antitumor, and human periodontal ligament fibroblast assays.
What they found
EPS yields 174.9–295.4 mg/l; DPPH scavenging 33.3–77% at 20 mg/ml; EPS5 most cytotoxic to MCF7/CaCo2/HepG2 and increased hPDLFC viability.
The limits
What it doesn't show
In vitro only—does not prove clinical cancer therapy or safe replacement of chemotherapeutics.
Key terms
- EPS
- Exopolysaccharides secreted by bacteria.
- LAB
- Lactic acid bacteria, often GRAS food microbes.
- DPPH assay
- In vitro radical-scavenging antioxidant test.
- IC50
- Concentration giving 50% cytotoxicity/inhibition.
- hPDLFCs
- Human periodontal ligament fibroblast cells.
- EPS 5
- EPS from L. delbrueckii ssp. bulgaricus DSM 20081T.
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Number of strains:
Common questions
How many strains?
10 (9 LAB, 1 Bb).
Highest EPS yield?
EPS5 ~295.4 mg/l.
EPS5 IC50s?
7.91 / 10.69 / 9.12 mg/ml (MCF7/CaCo2/HepG2).
Periodontal angle?
EPS5 increased hPDLFC viability.
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