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Cellulose plus C4 grass steers prairie soil microbes

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Adding cellulose and heat-tolerant Andropogon gerardii shifted restored prairie soils toward higher fungal biomass and slower-growing bacteria that may retain carbon.

Source

Soil microbial restoration strategies for promoting climate-ready prairie ecosystems

Docherty KM, Gutknecht JLM · Ecological applications : a publication of the Ecological Society of America · 2019

doi.org/10.1002/eap.1858Read the full paper ↗19 citationscc by

What they did

Agricultural soil in 72 pots received remnant-prairie inoculum and/or cellulose, planted with one vs three prairie species, then grown 62 days at ambient or +4°C; lipids, enzymes, 16S, and soil C were measured.

What they found

Cellulose raised fungal:bacterial lipid ratios (18:2ω6,9c and 18:1ω9c up; Gram-positive lipids down) regardless of plant mix. Plant richness had few microbial effects. Coupling cellulose with heat/drought-tolerant grasses selected higher fungal biomass and oligotroph-leaning bacteria.

The limits

What it doesn't show

A 62-day pot study cannot prove long-term field carbon sequestration; watering prevented drought so warming×moisture interactions were muted.

Key terms

Cellulose amendment
Recalcitrant carbon addition intended as a prebiotic to favor fungi and slow-growing bacteria.
PLFA
Phospholipid fatty acids used as biomass markers for fungi vs bacteria.
Copiotroph vs oligotroph
Fast labile-C users vs slow-growth taxa associated with C storage.
Andropogon gerardii
C4 big bluestem used as a heat-tolerant prairie grass.
Remnant inoculum
Microbes transferred from intact prairie soil into agricultural soil.

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Cellulose amendment mainly increased:

Common questions

What did cellulose do to fungi:bacteria?

Raised fungal:bacterial lipid ratios.

Did plant species richness strongly reshape microbes?

Few effects at ambient temperature.

Warming treatment?

+4°C above ambient for 62 days.

Restoration implication?

Pair cellulose with heat-tolerant grasses.

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