How do proteins find plasmodesmata?
PDLP1 is a type I membrane protein targeted to plasmodesmata; altering PDLP dosage changes GFP cell-to-cell trafficking.
Source
Specific targeting of a plasmodesmal protein affecting cell-to-cell communication
What they did
GFP-tagged cell-wall membrane proteins from Arabidopsis proteomes were localized across plant species; PDLP topology and knockout trafficking phenotypes were tested.
What they found
PDLP1 family proteins target plasmodesmata; PDLP1a has cytoplasmic C-tail and apoplastic DUF26 domains; combined KOs increase GFP movement.
The limits
What it doesn't show
Does not fully catalog every plasmodesmal protein or all cargoes regulated by PDLPs.
Key terms
- Plasmodesmata
- Cell-wall channels enabling plant symplastic continuity.
- PDLP1
- Plasmodesmata-located protein family member.
- Symplast
- Cytoplasmic continuum connected through plasmodesmata.
- Non-cell-autonomous
- Function in cells other than where a molecule was produced.
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Plasmodesmata are:
Common questions
What are plasmodesmata?
Channels crossing cell walls for intercellular communication.
PDLP1a topology?
Type I membrane protein: cytoplasmic C-tail, apoplastic DUF26.
KO phenotype?
Combined KOs increase GFP movement.
Why hard to study?
Embedded in rigid walls—hard to purify.
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