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How well does CA125 find ovarian cancer in GP care?

In UK primary care, CA125 ≥35 U/ml had high NPV but only about 10% PPV for ovarian cancer, and elevated values also flagged other cancers.

Source

The diagnostic performance of CA125 for the detection of ovarian and non-ovarian cancer in primary care: A population-based cohort study

Funston G, Hamilton W, Abel G, et al. · PLoS medicine · 2020

doi.org/10.1371/journal.pmed.1003295Read the full paper ↗102 citationscc by

What they did

Researchers studied 50,780 women who had CA125 tested in English primary care, linking results to cancer diagnoses. They estimated diagnostic accuracy at the conventional ≥35 U/ml cutoff and examined age-stratified performance and non-ovarian cancers.

What they found

Ovarian cancer incidence was 0.9%. At ≥35 U/ml, PPV was 10.1%, sensitivity 77%, specificity 93.8%, and AUC 0.92. Performance and cancer mix varied by age; elevated CA125 also related to non-ovarian cancers.

The limits

What it doesn't show

Observational EHR data cannot prove that changing CA125 thresholds or pathways improves survival, and symptom recording is incomplete.

Key terms

CA125
Cancer antigen 125 blood biomarker used to investigate possible ovarian cancer.
PPV
Positive predictive value—probability of disease given a positive test.
NPV
Negative predictive value—probability of no disease given a negative test.
Sensitivity
Proportion of true cases correctly identified by the test.
AUC
Area under the ROC curve summarizing discrimination across thresholds.
Primary care testing
Investigations ordered by general practitioners before specialist referral.

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In this primary-care cohort, PPV of CA125 ≥35 U/ml for ovarian cancer was about:

Common questions

What cutoff was evaluated?

The standard clinical cutoff of ≥35 U/ml.

Is a positive CA125 usually ovarian cancer?

No—PPV was about 10%, so most positives are not ovarian cancer.

Is a negative test reassuring?

NPV was very high (99.8%), though no test is perfect.

Why mention non-ovarian cancers?

Elevated CA125 can accompany other malignancies that clinicians should consider.

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