Immune transcriptome of bacteria-challenged sea bass

Study at a glance

Design

Animal / in-vitro — RNA-seq of head kidney/spleen from bacteria-challenged vs mock-challenged sea bass

N

Tissue RNA pooled from 15 fishes per preparation — no unpooled individual analytic N

Population

Lateolabrax japonicus (Japanese sea bass)

Outcome

Immune-related transcriptome after bacterial challenge

Structured fields used in claim comparison tables when every cited study has a complete layer.

How this study connects

Role on claims

Each row is a claim on a concept or method page where this paper supports, challenges, or qualifies the statement. Roles are hand-checked — not a model guess.

• SupportsInnate immunityconcept

  Multiple empirical papers in this library examine innate immunity with mechanistic biological findings.

  Evidence for the claim as stated.

• SupportsInnate immunityconcept

  Large transcriptome with many immune effectors and pathway annotations (hepcidin, lysozyme, RAG).

  Evidence for the claim as stated.

• SupportsInnate immunityconcept

  Systems and scales differ across innate immunity studies (species, tissues, methods), so mechanisms should not be over-generalised.

  Evidence for the claim as stated.

• SupportsRNA Sequencing (RNA-seq)method

  De novo assemblies turn RNA-seq into a catalogue for species without a finished genome. Illumina sequencing of Aloe vera root and leaf produced on the order of 43,000 CDS per tissue with annotations tied to secondary-metabolite pathways; bacteria-challenged sea bass head kidney and spleen yielded a large immune transcriptome including hepcidin, lysozyme and RAG annotations.

  Evidence for the claim as stated.

Open questions

Tensions this paper is part of

From concept pages' “where studies disagree.” Disagreement means the same question; scope means different assays, populations, or outcomes.

• Scope difference — different assays, populations, or outcomes

  SupportsInnate immunity

  Systems and scales differ across innate immunity studies (species, tissues, methods), so mechanisms should not be over-generalised.

  Also on this tension

  • Supports · Coral gene expression in white syndromes
  • Supports · How interferon throttles sterol synthesis in antiviral defense

Related papers in this topic

Same topic cluster — not a recommendation engine.

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• Was innate immunity already complex in early animals?
• How interferon throttles sterol synthesis in antiviral defense
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• Coral gene expression in white syndromes