Innate immunity
Immune transcriptome of bacteria-challenged sea bass
Open access · cc by · source: Europe PMC
Deep RNA-seq of bacteria-challenged Lateolabrax japonicus reveals innate immune genes.
How this study connects
Role on claims
Each row is a claim on a concept or method page where this paper supports, challenges, or qualifies the statement. Roles are hand-checked — not a model guess.
Multiple empirical papers in this library examine innate immunity with mechanistic biological findings.
Evidence for the claim as stated.
Large transcriptome with many immune effectors and pathway annotations (hepcidin, lysozyme, RAG).
Evidence for the claim as stated.
Systems and scales differ across innate immunity studies (species, tissues, methods), so mechanisms should not be over-generalised.
Evidence for the claim as stated.
De novo assemblies turn RNA-seq into a catalogue for species without a finished genome. Illumina sequencing of Aloe vera root and leaf produced on the order of 43,000 CDS per tissue with annotations tied to secondary-metabolite pathways; bacteria-challenged sea bass head kidney and spleen yielded a large immune transcriptome including hepcidin, lysozyme and RAG annotations.
Evidence for the claim as stated.
Open questions
Tensions this paper is part of
From concept pages' “where studies disagree.” Disagreement means the same question; scope means different assays, populations, or outcomes.
Systems and scales differ across innate immunity studies (species, tissues, methods), so mechanisms should not be over-generalised.
Study at a glance
- Design
- Animal / in-vitro — RNA-seq of head kidney/spleen from bacteria-challenged vs mock-challenged sea bass
- N
- Tissue RNA pooled from 15 fishes per preparation — no unpooled individual analytic N
- Population
- Lateolabrax japonicus (Japanese sea bass)
- Outcome
- Immune-related transcriptome after bacterial challenge
Structured fields used in claim comparison tables when every cited study has a complete layer.
Inspect source passages
Immune transcriptome of bacteria-challenged sea bass
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
Lateolabrax japonicus (sea bass).
Fish immunogenetics has received considerable attention due to its essential role in understanding the origin and evolution of immune systems [-].
Bacteria-challenged versus mock.
Further, it is also beneficial in the creation of immune-based therapy of severe fish diseases.
Key findings
Large transcriptome with many immune effectors and pathway annotations (hepcidin, lysozyme, RAG).
Methodology
RNA-seq of head kidney/spleen from bacteria- versus mock-challenged fish; assembled immune transcripts.
Limitations
Homology-based pathway calls; not a vaccine efficacy trial.
Related papers in this topic
Same topic cluster — not a recommendation engine.