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Gene expression

What do co-fermenting yeasts express together?

Shekhawat K, Patterton H, Bauer FF, et al. · BMC genomics · 2019

Open access · cc by · source: Europe PMC

In balanced continuous co-culture, L. thermotolerans contributes ~24% of anaerobic reads vs ~8% aerobically.

Study at a glance

Design
Animal / in-vitro — Continuous co-culture of S. cerevisiae and L. thermotolerans under anaerobic vs aerobic conditions
N
Two biological replicates per fermentation condition; species held near equal abundance ~24 h
Population
Mixed Saccharomyces cerevisiae and Lachancea thermotolerans cultures
Outcome
Condition-dependent transcriptome shares and mixing-responsive genes

Structured fields used in claim comparison tables when every cited study has a complete layer.

Key findings

L. thermotolerans transcript share is ~24% anaerobic vs ~8% aerobic, with anaerobic depth sufficient to recover mixing-responsive genes.

Methodology

Kept S. cerevisiae and L. thermotolerans near equal abundance via dilution rate and profiled mixed transcriptomes under anaerobic vs aerobic conditions.

Limitations

Imposed equal biomass is experimental; results are model-fermentation dependent.

How this study connects

Role on claims

Each row is a claim on a concept or method page where this paper supports, challenges, or qualifies the statement. Roles are hand-checked — not a model guess.

  • Time-of-day and mixed-culture design change the biological contrast RNA-seq is actually making. Tomato–DC3000 resistance was highest at 08:00 and susceptibility at 20:00, with red light the most disease-suppressive wavelength among those tested; in a chemostat co-culture, L. thermotolerans contributed about 24% of transcripts anaerobically versus about 8% aerobically.

    Evidence for the claim as stated.

  • How far a transcriptome may be generalised is itself in dispute in these papers. Tomato red-light findings are not automatically all crops; mixed-yeast dilution rates are experimental; LCL methylation–expression links may not hold in primary tissues; coral DEGs do not settle field etiology.

    Evidence for the claim as stated.

Open questions

Tensions this paper is part of

From concept pages' “where studies disagree.” Disagreement means the same question; scope means different assays, populations, or outcomes.

Related papers in this topic

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