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How does malaria’s blood-stage transcriptome unfold?

Plasmodium falciparum’s 48-hour intraerythrocytic cycle was profiled hourly, revealing tightly staged gene-expression programs after RBC invasion.

Source

The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum

Bozdech Z, Llinás M, Pulliam BL, et al. · PLoS biology · 2003

doi.org/10.1371/journal.pbio.0000005Read the full paper ↗1,259 citationscc by

What they did

Measured the IDC transcriptome of P. falciparum at 1-hour resolution across the ~48-hour cycle from merozoite invasion through ring/trophozoite/schizont stages.

What they found

Gene expression is highly periodic and stage-ordered across the IDC starting with merozoite invasion and parasitophorous vacuole formation.

The limits

What it doesn't show

Does not itself deliver a drug; focuses on blood-stage expression timing.

Key terms

IDC
Intraerythrocytic developmental cycle in RBCs.
Merozoite
Invasive blood-stage form that enters erythrocytes.
Parasitophorous vacuole
Host-derived vacuole housing the intracellular parasite.
Plasmodium falciparum
Major human malaria parasite.

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IDC length

Common questions

Cycle length?

~48 hours.

Resolution?

1-hour sampling.

Starts with?

Merozoite invasion of RBCs.

Why care?

Maps stage-specific expression for the pathogenic blood stage.

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