FLI1 circRNA FECR1 drives metastasis
Exonic circular RNA FECR1 binds the FLI1 promoter and epigenetically activates FLI1 in breast cancer.
Source
A novel FLI1 exonic circular RNA promotes metastasis in breast cancer by coordinately regulating TET1 and DNMT1
What they did
Used dCas9 chromatin immunoprecipitation (CasIP) in MDA-MB231 cells to pull RNAs at the FLI1 promoter, then tested FECR1 function and TET1/DNMT1 regulation.
What they found
FECR1 is a FLI1 exonic circRNA that activates FLI1 epigenetically and promotes invasive phenotypes; FLI1 is elevated in tumor vs adjacent tissue.
The limits
What it doesn't show
Not a clinical trial of circRNA therapy; human data are observational staining/expression.
Key terms
- Circular RNA
- Back-spliced covalently closed RNA isoform.
- FECR1
- FLI1 exonic circular RNA.
- FLI1
- ETS-family transcription factor oncogene.
- CasIP
- dCas9-guided chromatin RNA immunoprecipitation.
- TET1
- DNA demethylase involved in FECR1 regulation.
- DNMT1
- Maintenance DNA methyltransferase.
Flashcards
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FECR1 is:
Common questions
What is FECR1?
FLI1 exonic circular RNA.
Cell model?
MDA-MB231 breast cancer cells.
Epigenetic partners?
TET1 and DNMT1.
Phenotype?
Promotes metastasis-related programs.
More on Gene expression