FLI1 circRNA FECR1 drives metastasis

Open paper intelligence

Exonic circular RNA FECR1 binds the FLI1 promoter and epigenetically activates FLI1 in breast cancer.

Source

A novel FLI1 exonic circular RNA promotes metastasis in breast cancer by coordinately regulating TET1 and DNMT1

Chen N, Zhao G, Yan X, et al. · Genome biology · 2018

doi.org/10.1186/s13059-018-1594-y Read the full paper ↗355 citationscc by

Study at a glance

Design: Animal / in-vitro — dCas9 CasIP and functional assays of FECR1 circRNA in MDA-MB231 cells
N: Cell-line mechanistic study; no single primary analytic N in stored text
Population: MDA-MB231 breast cancer cells (with tumor vs adjacent FLI1 context)
Outcome: FECR1 activation of FLI1 and invasive phenotypes

Structured fields used in claim comparison tables when every cited study has a complete layer.

Inspect source passages

FLI1 circRNA FECR1 drives metastasis

These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.

1% formaldehyde used to fix CasIP complexes.

After puromycin selection, stable cells were treated with 1% formaldehyde to fix the dCas9-gRNA- FLI1 promoter chromatin complex.

FECR1 identity?

FECR1, a FLI1 exonic circular RNA that binds to the FLI1 promoter

Read passages in the original paper

What they did

Used dCas9 chromatin immunoprecipitation (CasIP) in MDA-MB231 cells to pull RNAs at the FLI1 promoter, then tested FECR1 function and TET1/DNMT1 regulation.

What they found

FECR1 is a FLI1 exonic circRNA that activates FLI1 epigenetically and promotes invasive phenotypes; FLI1 is elevated in tumor vs adjacent tissue.

The limits

What it doesn't show

Not a clinical trial of circRNA therapy; human data are observational staining/expression.

Key terms

Circular RNA: Back-spliced covalently closed RNA isoform.
FECR1: FLI1 exonic circular RNA.
FLI1: ETS-family transcription factor oncogene.
CasIP: dCas9-guided chromatin RNA immunoprecipitation.
TET1: DNA demethylase involved in FECR1 regulation.
DNMT1: Maintenance DNA methyltransferase.

Flashcards

1 / 10

0 of 10 answers reviewed

Research intelligence for this paper

See its role on concept claims, tensions it is part of, placement history, and related discoveries.

Open paper intelligence

Quiz yourself

1 / 7

FECR1 is:

Common questions

What is FECR1?

FLI1 exonic circular RNA.

Cell model?

MDA-MB231 breast cancer cells.

Epigenetic partners?

TET1 and DNMT1.

Phenotype?

Promotes metastasis-related programs.