Plant TIR domain binds viral elicitor
Tobacco N protein’s TIR domain is critical for association with TMV p50 elicitor.
Source
A novel role for the TIR domain in association with pathogen-derived elicitors
Study at a glance
- Design
- Animal / in-vitro — Co-expression domain mapping of N and TMV p50 in N. benthamiana
- N
- Plant molecular genetics / protein-association assays — no single sample N
- Population
- Nicotiana benthamiana expressing tagged N and TMV p50
- Outcome
- TIR-domain requirement for N–p50 association and HR cell death
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What they did
Tagged N and TMV p50, co-expressed them in N. benthamiana, and mapped domains needed for physical association and HR cell death.
What they found
Co-expression triggers HR in 2 days; N and p50 are cytoplasmic; the TIR domain of N is critical for association with p50.
The limits
What it doesn't show
Does not solve a full atomic structure of the N–p50 complex.
Key terms
- TIR domain
- Toll/interleukin-1 receptor homology domain.
- N protein
- Tobacco NB-LRR resistance protein against TMV.
- p50
- TMV replicase elicitor fragment.
- HR
- Hypersensitive response cell death.
- NB-LRR
- Nucleotide-binding leucine-rich repeat R protein.
- Elicitor
- Pathogen molecule recognized by host R proteins.
Flashcards
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Critical domain:
Common questions
Pathogen?
Tobacco mosaic virus (TMV).
HR timing?
About 2 days after co-infiltration.
Key domain?
TIR domain of N.
Localization?
Cytoplasmic.
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