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Which sensor starts IFN against SARS-CoV-2 in lung cells?

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SARS-CoV-2 triggers delayed IFN mainly via MDA5/LGP2 sensing of viral intermediates, with IRF3/IRF5/NF-κB p65 as key TFs.

Source

MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells

Yin X, Riva L, Pu Y, et al. · Cell reports · 2021

doi.org/10.1016/j.celrep.2020.108628Read the full paper ↗354 citationscc by

Study at a glance

Design
Animal / in-vitro — Lung epithelial infection + RNA-sensor/TF screens
N
N=16 · 16 putative sensors screened
Population
Lung epithelial cells infected with SARS-CoV-2
Outcome
Sensors/TFs governing IFN induction

Structured fields used in claim comparison tables when every cited study has a complete layer.

What they did

Infected lung epithelial cells and screened candidate RNA sensors and transcription factors for IFN induction.

What they found

Delayed IFN; MDA5 and LGP2 primary sensors; viral intermediates activate MDA5 pathway; IRF3, IRF5, NF-κB/p65 key TFs.

The limits

What it doesn't show

In-vitro epithelial circuits may differ from whole-lung immune networks in patients.

Key terms

MDA5
Cytosolic RNA sensor central to IFN induction here.
LGP2
RIG-I-like receptor co-regulator of sensing.
IFN response
Interferon antiviral program.
Viral intermediates
Replication-derived RNAs triggering MDA5.
IRF3/IRF5
Interferon regulatory transcription factors.
NF-κB/p65
Inflammatory TF subunit highlighted here.

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Primary sensors:

Common questions

Main sensors?

MDA5 and LGP2.

IFN timing?

Delayed.

Sensors screened?

16.

Key TFs?

IRF3, IRF5, NF-κB/p65.

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