What marks definitive endoderm progenitors in hESCs?

Single-cell RNA-seq separates definitive endoderm progenitors and links metabolism/hypoxia to DE differentiation.

Source

Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm

Chu LF, Leng N, Zhang J, et al. · Genome biology · 2016

What they did

Profiled lineage-specific progenitors from H1 hESCs by scRNA-seq and tested oxygen conditions during DE differentiation.

PC5 separates DE cells; metabolism genes load on that axis; severe hypoxia (1.5% O2) increases CXCR4+ DE-like cells versus higher O2.

The limits

Does not provide a full clinical protocol for organ replacement therapy.

Key terms

Definitive endoderm (DE): Embryonic gut endoderm progenitors from the primitive streak
scRNA-seq: Transcriptomes of individual cells
CXCR4: Surface marker enriched in DE progenitors
Hypoxia: Low oxygen culture condition

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PC separating DE cells

Common questions

What lineage is DE?

Internal embryonic gut layer precursors

What separates DE in PCA?

PC5

Does hypoxia help DE?

1.5% O2 increases CXCR4+ cells

System?

Human ES cell differentiation