What marks definitive endoderm progenitors in hESCs?
Single-cell RNA-seq separates definitive endoderm progenitors and links metabolism/hypoxia to DE differentiation.
Source
Single-cell RNA-seq reveals novel regulators of human embryonic stem cell differentiation to definitive endoderm
Study at a glance
- Design
- Animal / in-vitro — scRNA-seq of H1/H9 progenitors and oxygen-condition tests during definitive endoderm differentiation
- N
- N=1018 · 1,018 single cells analysed (including H1 n=212, H9 n=162, HFFs n=159)
- Population
- Human embryonic stem cells and lineage-specific progenitors
- Outcome
- Single-cell regulators and hypoxia effects on definitive endoderm differentiation
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What marks definitive endoderm progenitors in hESCs?
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
What is definitive endoderm?
definitive endoderm (DE) is the internal layer of the embryonic gut, formed by the recruitment of epiblast cells through the primitive streak.
Do mouse studies agree on hypoxia and DE?
hypoxia culture facilitated DE differentiation [].
What they did
Profiled lineage-specific progenitors from H1 hESCs by scRNA-seq and tested oxygen conditions during DE differentiation.
What they found
PC5 separates DE cells; metabolism genes load on that axis; severe hypoxia (1.5% O2) increases CXCR4+ DE-like cells versus higher O2.
The limits
What it doesn't show
Does not provide a full clinical protocol for organ replacement therapy.
Key terms
- Definitive endoderm (DE)
- Embryonic gut endoderm progenitors from the primitive streak
- scRNA-seq
- Transcriptomes of individual cells
- CXCR4
- Surface marker enriched in DE progenitors
- Hypoxia
- Low oxygen culture condition
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Quiz yourself
PC separating DE cells
Common questions
What lineage is DE?
Internal embryonic gut layer precursors
What separates DE in PCA?
PC5
Does hypoxia help DE?
1.5% O2 increases CXCR4+ cells
System?
Human ES cell differentiation
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