Atlantic cod heat-shock gene responses

Heat shock elevates cortisol and rewires chaperone and stress-gene expression in Atlantic cod tissues.

Source

Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques

Hori TS, Gamperl AK, Afonso LO, et al. · BMC genomics · 2010

doi.org/10.1186/1471-2164-11-72 Read the full paper ↗64 citationscc by

Study at a glance

Design: Animal / in-vitro — Heat-shocked Atlantic cod; SSH libraries from liver/head kidney/muscle with QPCR validation
N: SSH pools used 32 fish per tissue/treatment group; QPCR used 6 individuals per treatment/time — no single primary N
Population: Atlantic cod (Gadus morhua)
Outcome: Heat-shock–responsive transcripts and plasma cortisol response

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Atlantic cod heat-shock gene responses

These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.

Atlantic cod (Gadus morhua).

Temperatures are known to vary considerably at aquaculture cage-sites [] and can approach upper critical temperatures (i.e. temperatures that are lethal) for Atlantic cod ( Gadus morhua ).

Plasma cortisol.

Cortisol is often regarded as a suitable indicator of stress, and one of the key hormones regulating the stress response

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What they did

Heat-shocked Atlantic cod, measured plasma cortisol, built SSH libraries from liver/head kidney/muscle, and validated candidates by QPCR.

What they found

Cortisol rose sharply after heat shock (e.g., ~132–165 ng/ml peaks) while controls stayed basal; many chaperone/stress transcripts were confirmed heat-responsive.

The limits

What it doesn't show

Cage aquaculture context may differ from wild thermal regimes; not every SSH clone was functionally proven.

Key terms

Heat shock: Acute high-temperature stress.
Cortisol: Primary stress hormone readout.
SSH: Suppression subtractive hybridization for DE transcripts.
QPCR: Quantitative PCR validation of candidates.
HSP: Heat-shock protein chaperone family.
Atlantic cod: Gadus morhua study species.

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Common questions

Species?

Atlantic cod.

Stress?

Heat shock.

Hormone?

Cortisol rises.

Method?

SSH + QPCR.