Atlantic cod heat-shock gene responses
Heat shock elevates cortisol and rewires chaperone and stress-gene expression in Atlantic cod tissues.
Source
Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques
What they did
Heat-shocked Atlantic cod, measured plasma cortisol, built SSH libraries from liver/head kidney/muscle, and validated candidates by QPCR.
What they found
Cortisol rose sharply after heat shock (e.g., ~132–165 ng/ml peaks) while controls stayed basal; many chaperone/stress transcripts were confirmed heat-responsive.
The limits
What it doesn't show
Cage aquaculture context may differ from wild thermal regimes; not every SSH clone was functionally proven.
Key terms
- Heat shock
- Acute high-temperature stress.
- Cortisol
- Primary stress hormone readout.
- SSH
- Suppression subtractive hybridization for DE transcripts.
- QPCR
- Quantitative PCR validation of candidates.
- HSP
- Heat-shock protein chaperone family.
- Atlantic cod
- Gadus morhua study species.
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Topic:
Common questions
Species?
Atlantic cod.
Stress?
Heat shock.
Hormone?
Cortisol rises.
Method?
SSH + QPCR.
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