Nanomaterials
NH4OH tunes silica dots from 20 to 500 nm
Open access · cc by · source: Europe PMC
Varying ammonia in a Stöber synthesis sizes silica nanoparticles from 20 to 200 nm (then 500 nm with extra TEOS), enabling core–shell TAMRA–BHQ2 quenching studies over unusually long distances.
Key findings
NH4OH (0.3–5.0 mL) sizes particles 20–200 nm; extra TEOS grows them to 500 nm. TEOS or ethanol volume barely changes diameter (~145 or 70 nm). Quenching extends farther than classical Förster expectations.
Methodology
Authors mapped SNP diameter versus NH4OH, TEOS and ethanol, covalently doped dyes via APTES, grew silica shells, conjugated BHQ2, and recorded TAMRA quenching versus quencher loading and shell thickness.
Limitations
The ‘greater than expected’ distance is not turned into a calibrated molecular ruler for a biological analyte.
How this study connects
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