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Circadian biology

Does the plant clock gate auxin responses?

Covington MF, Harmer SL · PLoS biology · 2007

Open access · cc by · source: Europe PMC

The Arabidopsis circadian clock regulates auxin-responsive transcription and gates growth responses to auxin.

Study at a glance

Design
Animal / in-vitro — Circadian transcriptomes overlapped with auxin responses; timed auxin transcriptional/growth assays
N
Arabidopsis seedling genome-wide profiling — no single organismal N in stored text
Population
Arabidopsis thaliana seedlings
Outcome
Clock gating of auxin transcriptional responses and growth

Structured fields used in claim comparison tables when every cited study has a complete layer.

Key findings

>10% of seedling genes cycle; auxin-induced genes are enriched for clock regulation; endogenous/exogenous auxin transcriptional responses and auxin-driven growth are clock-gated.

Methodology

Profiled circadian transcriptomes and tested overlaps with auxin-regulated genes, then assayed transcriptional and growth responses to auxin across the day.

Limitations

Does not fully map every clock–auxin molecular node in all tissues or prove clinical relevance outside plants.

How this study connects

Role on claims

Each row is a claim on a concept or method page where this paper supports, challenges, or qualifies the statement. Roles are hand-checked — not a model guess.

  • Arabidopsis circadian transcriptomes overlapped auxin-regulated genes: more than 10% of seedling genes cycle, auxin-induced genes are enriched for clock regulation, and both transcriptional responses to auxin and auxin-driven growth are clock-gated. Luciferase or transcriptional reporters across the day are how gating is scored; every clock–auxin node in every tissue is not mapped.

    Evidence for the claim as stated.

  • Clock-gated auxin reporters and Wnt5a TCF reporters both measure transcription, but one asks 'what time of day' (>10% of seedling genes cycle) and the other asks 'which Frizzled is present'. The same luminescence number cannot be compared across a plant growth assay and a mammalian β-catenin pathway.

    Evidence for the claim as stated.

Open questions

Tensions this paper is part of

From concept pages' “where studies disagree.” Disagreement means the same question; scope means different assays, populations, or outcomes.

  • Scope difference — different assays, populations, or outcomes

    Clock-gated auxin reporters and Wnt5a TCF reporters both measure transcription, but one asks 'what time of day' (>10% of seedling genes cycle) and the other asks 'which Frizzled is present'. The same luminescence number cannot be compared across a plant growth assay and a mammalian β-catenin pathway.

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Same topic cluster — not a recommendation engine.