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Infant diet shapes gut–host immunity

Breast- vs formula-fed infants differ in gut microbiota and host immune gene expression.

Source

A metagenomic study of diet-dependent interaction between gut microbiota and host in infants reveals differences in immune response

Schwartz S, Friedberg I, Ivanov IV, et al. · Genome biology · 2012

doi.org/10.1186/gb-2012-13-4-r32Read the full paper ↗169 citationscc by

What they did

Sequenced stool metagenomes and profiled host epithelial mRNA from 6 breast-fed and 6 formula-fed infants, then correlated taxa/genes with host transcripts.

What they found

Diet altered Firmicutes/Actinobacteria composition; 459 intestinal and 660 immunity genes were tested, with many differentially expressed between diets.

The limits

What it doesn't show

Small n; association does not prove which microbes cause each transcript change.

Key terms

Metagenome
Collective microbial DNA from a community.
Breast-fed (BF)
Infants fed human milk.
Formula-fed (FF)
Infants fed formula.
Host transcriptome
Gene expression of shed gut epithelial cells.
Actinobacteria
Phylum often enriched in infant guts.
FDR
False discovery rate multiple-testing control.

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BF/FF n:

Common questions

Infants per diet?

Six BF and six FF.

Intestinal gene set size?

459.

Immunity gene set size?

660.

Key contrast?

Diet-linked microbiota and host immune transcripts.

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