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Tiling arrays reveal plant circadian RNAs

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Genome tiling arrays uncover extensive circadian-regulated transcription across the Arabidopsis genome.

Source

Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays

Hazen SP, Naef F, Quisel T, et al. · Genome biology · 2009

doi.org/10.1186/gb-2009-10-2-r17Read the full paper ↗93 citationscc by

What they did

Sampled a 2-day circadian time course at 4-hour resolution on whole-genome tiling arrays.

What they found

Known clock genes cycle as expected; many additional exons/ncRNA features show circadian rhythms.

The limits

What it doesn't show

Two-day series limits period precision versus longer time courses.

Key terms

Circadian clock
~24 h endogenous timing system.
Tiling array
Dense probes across the genome.
Arabidopsis
Plant model organism.
PRR genes
PSEUDO-RESPONSE REGULATOR clock genes.
Spectral analysis
Tests rhythmic hybridization.
ncRNA
Noncoding RNA features.

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Common questions

Organism?

Arabidopsis.

Resolution?

4 h.

Duration?

2 days.

Platform?

Tiling arrays.

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