What do co-fermenting yeasts express together?
In balanced continuous co-culture, L. thermotolerans contributes ~24% of anaerobic reads vs ~8% aerobically.
Source
RNA-seq based transcriptional analysis of Saccharomyces cerevisiae and Lachancea thermotolerans in mixed-culture fermentations under anaerobic conditions
Study at a glance
- Design
- Animal / in-vitro — Continuous co-culture of S. cerevisiae and L. thermotolerans under anaerobic vs aerobic conditions
- N
- Two biological replicates per fermentation condition; species held near equal abundance ~24 h
- Population
- Mixed Saccharomyces cerevisiae and Lachancea thermotolerans cultures
- Outcome
- Condition-dependent transcriptome shares and mixing-responsive genes
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What do co-fermenting yeasts express together?
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
L. thermotolerans transcripts were 24% of anaerobic mixed-culture reads.
L. thermotolerans transcripts accounted for 24% of the sequences reads in anaerobic mixed cultures and ≈ 8% in aerobic mixed cultures.
Anaerobic L. thermotolerans read share?
accounted for 24% of the sequences reads in anaerobic mixed cultures and ≈ 8% in aerobic mixed cultures.
What they did
Kept S. cerevisiae and L. thermotolerans near equal abundance via dilution rate and profiled mixed transcriptomes under anaerobic vs aerobic conditions.
What they found
L. thermotolerans transcript share is ~24% anaerobic vs ~8% aerobic, with anaerobic depth sufficient to recover mixing-responsive genes.
The limits
What it doesn't show
Imposed equal biomass is experimental; results are model-fermentation dependent.
Key terms
- Mixed culture
- Two yeasts grown together.
- Continuous culture
- Dilution-controlled steady growth.
- Anaerobic fermentation
- Fermentation without oxygen.
- RNA-seq
- Transcript quantification by sequencing.
- L. thermotolerans
- Non-Saccharomyces wine yeast partner.
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Anaerobic share:
Common questions
Anaerobic read share?
~24% L. thermotolerans.
Aerobic share?
~8%.
How balanced?
Dilution rates for ~24 h.
Partner?
S. cerevisiae.
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