What do co-fermenting yeasts express together?
In balanced continuous co-culture, L. thermotolerans contributes ~24% of anaerobic reads vs ~8% aerobically.
Source
RNA-seq based transcriptional analysis of Saccharomyces cerevisiae and Lachancea thermotolerans in mixed-culture fermentations under anaerobic conditions
What they did
Kept S. cerevisiae and L. thermotolerans near equal abundance via dilution rate and profiled mixed transcriptomes under anaerobic vs aerobic conditions.
What they found
L. thermotolerans transcript share is ~24% anaerobic vs ~8% aerobic, with anaerobic depth sufficient to recover mixing-responsive genes.
The limits
What it doesn't show
Imposed equal biomass is experimental; results are model-fermentation dependent.
Key terms
- Mixed culture
- Two yeasts grown together.
- Continuous culture
- Dilution-controlled steady growth.
- Anaerobic fermentation
- Fermentation without oxygen.
- RNA-seq
- Transcript quantification by sequencing.
- L. thermotolerans
- Non-Saccharomyces wine yeast partner.
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Anaerobic share:
Common questions
Anaerobic read share?
~24% L. thermotolerans.
Aerobic share?
~8%.
How balanced?
Dilution rates for ~24 h.
Partner?
S. cerevisiae.
More on Gene expression