LN DCs shelter antibiotic-tolerant Salmonella

Slow-growing Salmonella in cecum lymph-node dendritic cells survive ciprofloxacin therapy.

Source

Cecum lymph node dendritic cells harbor slow-growing bacteria phenotypically tolerant to antibiotic treatment

Kaiser P, Regoes RR, Dolowschiak T, et al. · PLoS biology · 2014

What they did

Infected mice with S. Typhimurium, treated with high-dose ciprofloxacin, and localized surviving bacteria to cLN dendritic cells.

Gut lumen clears in 3 h, but 50–1,000 bacteria persist in cLN up to 10 days with slowed killing after the first 2 h.

The limits

Does not invent a new antibiotic; mouse model of persistence.

Key terms

Phenotypic tolerance: Noninherited antibiotic survival of susceptible bacteria.
Persister: Slow-growing tolerant subpopulation.
cLN: Cecal lymph node.
Dendritic cell: Antigen-presenting immune cell harboring bacteria.
Ciprofloxacin: Fluoroquinolone antibiotic used here.
MIC: Minimal inhibitory concentration.

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Lumen clear by:

Common questions

Lumen clearance time?

Within 3 h.

cLN survivors?

50–1,000 for up to 10 d.

Early kill?

10-fold drop in first 2 h.

Niche?

Dendritic cells in cLN.