LN DCs shelter antibiotic-tolerant Salmonella
Slow-growing Salmonella in cecum lymph-node dendritic cells survive ciprofloxacin therapy.
Source
Cecum lymph node dendritic cells harbor slow-growing bacteria phenotypically tolerant to antibiotic treatment
Study at a glance
- Design
- Animal / in-vitro — S. Typhimurium–infected mice treated with high-dose ciprofloxacin; residual bacteria localized to cLN DCs
- N
- Mouse infection/antibiotic kinetics study; infection dose 5×10⁷ cfu — no single analytic N in stored text
- Population
- Mice infected with Salmonella Typhimurium
- Outcome
- Persistence of slow-growing bacteria in cecum lymph-node dendritic cells after ciprofloxacin
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Inspect source passages
LN DCs shelter antibiotic-tolerant Salmonella
These passages occur verbatim in the stored paper text. They support the points listed below; they do not verify every statement in the explanation.
Pathogen cleared from gut lumen within 3 h.
Within 3 h of ciprofloxacin treatment, the pathogen was cleared from the gut lumen and pathogen spread to the spleen was prevented by the antibiotic ( and ).
50–1,000 viable bacteria remained up to 10 d.
After the first 2 h, the killing kinetics slowed down and 50–1,000 viable bacteria remained for up to 10 d (, ).
What they did
Infected mice with S. Typhimurium, treated with high-dose ciprofloxacin, and localized surviving bacteria to cLN dendritic cells.
What they found
Gut lumen clears in 3 h, but 50–1,000 bacteria persist in cLN up to 10 days with slowed killing after the first 2 h.
The limits
What it doesn't show
Does not invent a new antibiotic; mouse model of persistence.
Key terms
- Phenotypic tolerance
- Noninherited antibiotic survival of susceptible bacteria.
- Persister
- Slow-growing tolerant subpopulation.
- cLN
- Cecal lymph node.
- Dendritic cell
- Antigen-presenting immune cell harboring bacteria.
- Ciprofloxacin
- Fluoroquinolone antibiotic used here.
- MIC
- Minimal inhibitory concentration.
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Lumen clear by:
Common questions
Lumen clearance time?
Within 3 h.
cLN survivors?
50–1,000 for up to 10 d.
Early kill?
10-fold drop in first 2 h.
Niche?
Dendritic cells in cLN.
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