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How open is the Listeria pan-genome?

Eleven new genomes spanning serotypes show a stable but open pan-genome with nine hypervariable hotspots dominated by mobile elements.

Source

Reassessment of the Listeria monocytogenes pan-genome reveals dynamic integration hotspots and mobile genetic elements as major components of the accessory genome

Kuenne C, Billion A, Mraheil MA, et al. · BMC genomics · 2013

doi.org/10.1186/1471-2164-14-47Read the full paper ↗180 citationscc by

What they did

Extended L. monocytogenes genome sets by 11 strains covering all serotypes and reassessed pan-genome structure.

What they found

The pan-genome is highly stable but not closed; accessory genes concentrate in hypervariable hotspots with mobile genetic elements as major accessory components.

The limits

What it doesn't show

Gene presence/absence aids diagnostics/phylogenetics but does not alone prove each virulence phenotype.

Key terms

Pan-genome
All genes found across strains of a species.
Accessory genome
Genes present in only some strains.
Hypervariable hotspot
Region with frequent gene content change.
Mobile genetic element
DNA that moves between genomes.
Serotype
Strain group defined by surface antigens.

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Pan-genome:

Common questions

Closed pan-genome?

No—stable but not closed.

Strains added?

11.

Hotspots?

Nine hypervariable hotspots.

Accessory drivers?

Mobile genetic elements.

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