Apical-out enteroids for pathogen access
Suspending human enteroids without ECM flips polarity so microbes can meet the apical face.
Source
Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions
What they did
Human enteroids were cultured in suspension without BME; β1-integrin roles in polarity were tested.
What they found
Polarity reversed within hours; β1 integrin oriented polarity for apical pathogen assays.
The limits
What it doesn't show
Not a clinical infection trial; enteroids only approximate gut immunity.
Key terms
- Enteroid
- 3D intestinal stem-cell culture.
- Apical-out
- Apical membrane faces medium.
- β1 integrin
- ECM receptor steering polarity.
- BME
- Basement-membrane extract scaffold.
- Polarity
- Apical vs basolateral organization.
- Host–pathogen
- Microbe–epithelium interaction.
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Topic:
Common questions
How reverse?
Suspension without ECM.
Key receptor?
β1 integrin.
Why useful?
Apical microbe access.
Species?
Human.
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